| 靳雷高杰刘云燕马琼杨彤涛裘秀春范清宇马保安.microRNA-194模拟物对成骨肉瘤细胞系SOSP_9607生物学
特性的影响[J].,2011,11(4):615-617 |
| microRNA-194模拟物对成骨肉瘤细胞系SOSP_9607生物学
特性的影响 |
| Effects of MicroRNA-194 mimics on Proliferation and Apoptosisof Human Osteosarcoma Cell Line SOSP-9607 |
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| DOI： |
| 中文关键词: 骨肉瘤 成骨肉瘤细胞系SOSP_9607 miR-194 细胞增殖 细胞凋亡 |
| 英文关键词: Osteosarcoma SOSP-9607 cell line miR-194 Cell proliferation Cell apoptosis |
| 基金项目:基金项目国家自然科学基金项目( No.30672143) |
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| 中文摘要: |
| 目的：观察miR-194 模拟物对于成骨肉瘤细胞系SOSP_9607 细胞增殖、周期和凋亡的影响。方法：四甲基偶氮唑蓝（MTT）
法绘制细胞生长曲线, 流式细胞仪测定细胞凋亡和周期。将SOSP_9607细胞分为对照组和实验组,对照组分为阴性对照和正常细
胞对照组。实验组采用miR-194 模拟物（hsa-miR-194 mimics）转染成骨肉瘤细胞系SOSP_9607，增强SOSP_9607细胞内miR-194
的活性。结果：与对照组比较,实验组细胞的增殖能力明显下降。实验组凋亡率（10.1±0.22）%与阴性对照组凋亡率（3.3±0.19）%相
比明显增高(P<0.01)。与对照组比较，实验组细胞周期G0/G1细胞比例显著增加,G2/M期细胞比例显著减少，S期细胞比例显著减
少(P<0.01)。结论：通过转染miR-194 模拟物增强SOSP_9607细胞中miR-194 的活性对SOSP_9607 细胞的增殖和凋亡造成显著
影响。 |
| 英文摘要: |
| Objective: To investigate the effect of microRNA-194 mimics on proliferation, cell cycle and apoptosis of human osteosarcoma
cell line SOSP-9607. Methods: MTT assay was used to detect cell growth. FCM was employed to detect cell apoptosis rate
and cell cycle analysis. Cultured SOSP-9607 cells were divided into three groups:negtive control group, blank group and experimental
group. In experimental group, hsa-miR-194 mimics were used to increase the activity of miR -194. Results: Hsa-miR-194 mimics inhibited
SOSP-9607 cell proliferation, while the control group had no obvious effect on cell proliferation. The apoptosis ratio of the experimental
group[（10.1±0.22）%] was obviously higher than that of the control group[（3.3±0.19）%]（P<0.01）. Hsa-miR-194 mimics increased
the cell ratio of G0/G1 phase, simultaneously decreased significantly the cell ratios of G2/M phase and S phase.( P<0.01). Conclusion: |
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