| 戎嵘韦红梅周静朱俊东.大豆异黄酮激活PPARγ抑制人乳腺癌MCF-7 细胞增殖[J].,2011,11(6):1025-1029 |
| 大豆异黄酮激活PPARγ抑制人乳腺癌MCF-7 细胞增殖 |
| Soy Isoflavones Inhibit the Proliferation of Human Breast Cancer MCF-7Cells via the Activation of PPARγ |
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| DOI： |
| 中文关键词: 大豆异黄酮 乳腺癌细胞 PPARγ 增殖 |
| 英文关键词: Soy isoflavones breast cancer cells PPARγ proliferation |
| 基金项目:国家自然科学基金（No.30771793） |
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| 中文摘要: |
| 目的：探讨两种大豆异黄酮主要成分染料木黄酮（genistein,GEN）和大豆苷元（daidzein,DAI）抑制人乳腺癌MCF-7 细胞增殖
的作用与过氧化物酶体增殖激活物受体γ(peroxisome proliferators-activated receptorγ, PPARγ)信号途径的关系。方法：采用免
疫细胞化学染色方法观察MCF-7 细胞的PPARγ 表达情况，PPARγ 介导的荧光素酶报告基因检测大豆异黄酮和PPARγ 配体
罗格列酮（rosiglitazone,ROS）对MCF-7 细胞PPARγ 的激活作用，MCF-7 细胞分别经8×10-5mol/L GEN、DAI 和1×10-5mol/L 的
ROS 单独或联合1×10-5mol/L 的PPARγ 特异性抑制剂GW9662 联合处理24、48 和72 h 后，用CCK-8 法检测细胞增殖。结果：
MCF-7 细胞存在有PPARγ 表达，GEN、DAI 呈剂量依赖性增强报告基因荧光素酶活性，且这种作用可被GW9662 明显阻断；
GEN、DAI 和ROS 呈时间依赖性明显抑制MCF-7 细胞增殖（P＜0.05），而GW9662 可以显著削弱GEN、DAI 和ROS 对MCF-7 细
胞的增殖抑制作用（P＜0.05）。结论：大豆异黄酮可通过激活乳腺癌MCF-7 细胞的PPARγ信号途径抑制其增殖。 |
| 英文摘要: |
| Objective: To investigate the effect of two major components of soy isoflavones genistein,daidzein and peroxisome on
the proliferation of human breast cancer MCF-7 cells, and the relationship between it and the proliferators-activated receptor γ(PPARγ)
signal pathway. Methods: The expression of PPARγ in breast cancer MCF-7 cells was detected by immunocytochemical staining, and
the activation of PPARγ in MCF-7 cells induced by isoflavones was measured by luciferase reporter gene assay. The proliferation of
MCF-7 cells was measured by CCK-8 assay. Results: There was expression of PPARγ in breast cancer MCF-7 cells. The luciferase activities
of MCF-7 cells tranfected with luciferase reporter gene plasmid showed a dose-response relationship with genistein and daidzein
treatment. GW9662 significantly inhibited the expression of reporter gene induced by genistein and daidzein. Genistein, daidzein and
rosiglitazone inhibited the proliferation of cancer cells MCF-7in a time-dependent manner （P＜0.05）, but the inhibitory effects of genistein,
daidzein and rosiglitazone on the proliferation of cancer cells could be blocked by GW9662 significantly （P＜0.05）. Conclusions:
Soy isoflavones inhibit the proliferation of breast cancer MCF-7 cells by activating the PPARγsignal pathway. |
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