| 马晓蕾潘峰胡丹.视神经损伤后视网膜Müller 细胞未折叠蛋白反应
相关因子IRE-1 与GLAST 的表达[J].,2011,11(8):1447-1450 |
| 视神经损伤后视网膜Müller 细胞未折叠蛋白反应
相关因子IRE-1 与GLAST 的表达 |
| Expression of Unfolded Protein Reaction Related Factor IRE-1 and GLASTin Retinal Müller Cell after Optic Nerve Injury |
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| DOI： |
| 中文关键词: 未折叠蛋白反应 Müller 细胞 GLAST 视网膜 大鼠 |
| 英文关键词: UPR Müller cells GLAST Retina Rat |
| 基金项目:国家自然科学基金（30772368） |
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| 中文摘要: |
| 目的：研究视神经损伤后视网膜Müller 细胞中是否有未折叠蛋白反应（UPR）及其与L 一谷氨酸／L 一天门冬氨酸转运体
（GLAST）的关系。方法：视神经钳夹伤模型建立成功后，运用HE 染色观察视网膜神经节细胞数目改变，免疫化学染色，免疫荧光
双标记，western-blot 观察UPR 相关因子需肌醇酶1 （IRE-1） 与GLAST 的表达及相关性。结果：视神经钳夹伤后IRE-1 与
GLAST 在视网膜Muller 细胞上共表达，，术后第一天前呈上升趋势，在第一天达到顶峰，后呈下降趋势，第七天下降明显。结论：
视神经损伤后，IRE-1 与GLAST 的趋势变化有一定相关性，提示未折叠蛋白反应可能是调控GLAST 变化的原因之一。 |
| 英文摘要: |
| Objective: To investigate the unfolded protein response （UPR）in retinal Müller cells after optic nerve injury and the
relationship between UPR and glutamate/aspartate transporter (GLAST). Methods: The change of the amount of retina gangliocyte was
detected by HE dying and the expression and relevance of UPR correlation factor-inositol requiring enzyme 1 (IRE-1)and GLAST were
detected by immunohistochemical staining, immunofluorescent double-tagging, western-blot after the modeling of trapped wound in
optic nerve nipper. Results：IRE-1 and GLAST co-express in retinal Müller cells, ascend in the common trend and reach the summit one
day after surgery, then drop sharply in the seventh day.Conclusions: There was relationship between the IRE-1 and GLAST in trend
changes after optic nerve injured which suggests UPR may be one cause of the regulation of GLAST changes. |
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