| 程元星1 段晓明2△ 曾治中1 黄璐1 贺修胜3.转hGM-CSF 基因HepG2 细胞疫苗侵袭性和生长活性初步检测[J].,2011,11(9):1621-1624 |
| 转hGM-CSF 基因HepG2 细胞疫苗侵袭性和生长活性初步检测 |
| Initial Detection of Invasion and Growth Activity of hGM-CSF GeneTransfected HepG2 Cell Vaccine |
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| DOI： |
| 中文关键词: hGM-CSF 基因 Transwell 体外侵袭 HepG2 肝癌疫苗 |
| 英文关键词: hGM-CSF gene Transwell in vitro invasion HepG2 liver cancer vaccines |
| 基金项目:湖南省卫生厅科研基金资助项目(B2005-178) |
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| 中文摘要: |
| 目的：探讨经60Co 处理的转hGM-CSF 基因的HepG2 肝癌疫苗的侵袭性和生长活性变化。方法：体外培养三种肝癌细胞
（①野生型HepG2 肝癌细胞②转染hGM-CSF 基因的HepG2 肝癌细胞③60Co 射线处理的转hGM-CSF 基因的HepG2 肝癌疫苗）
采用MTT 方法检测三种细胞在24h、48h、72h 的OD 值并绘出生长曲线；利用transwell 小室进行体外侵袭实验来观察上述三种
细胞侵袭性；用RT-PCR 技术检测上述三种细胞基质金属蛋白酶2（MMP-2）在mRNA 水平上表达的变化；结果：经60Co 照射处
理的转hGM-CSF 基因的HepG2 肝癌疫苗组OD 值在相同培养时间点较其他两组明显变小且差异有显著性(P<0.05)。三种细胞
（上述①②③种细胞）transwell 侵袭试验显示：③组穿过人造基底膜的细胞数量明显少于前两组；PT-PCR 示：③组细胞的MMP-2
的mRNA 的表达明显低于①②。结论：经过60Co 处理过的转hGM-CSF 基因的HepG2 肝癌疫苗的侵袭性和生长活性均明显降
低。 |
| 英文摘要: |
| Objective: To investigate the invasion and growth of HepG2 liver cancer vaccines transferred by hGM-CSF gene and
which be treated by Co60. Methods: Three hepatoma cells were cultured in vitro (①wild-type HepG2 hepatoma cells②HepG2 hepatoma
cells transfected by hGM-CSF gene ③ liver cancer vaccines HepG2 transfected by hGM-CSF gene and irradiated by 60Co). The OD
values of three types of cells cultured at 24h, 48h, 72h was detected by MTT (① ② ③ these kinds of cells) and the growth curve was
ploted . The three cell invasion was detected by using in vitro transwell chamber invasion assay; The expression of three cell matrix metalloproteinase
2 (MMP-2) was detected by RT-PCR; Results: 60Co irradiation of hGM-CSF gene transfected liver cancer vaccines
HepG2, the growth activity of culture at the same time was significantly lower than that of the other two groups and the difference was
significant (P <0.05). Three kinds of cell (①②③ these kinds of cells) transwell invasion test showed: ③ group the number of cells
through artificial basement membrane was significantly less than that of the first two groups; ③ group of cells in the MMP-2 mRNA
was significantly lower than that of① ②group. Conclusion: The invasion of HepG2 liver cancer vaccines transferred by hGM-CSF gene
and treated by 60Co reduced significantly. |
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