| 张春香1 罗学刚2 童锦禄3 蒋声海1 符玉梅1 张涛1 杨道文1 吉春花1.牛肠激酶催化亚基工程菌培养条件的探索与鉴定[J].,2011,11(9):1691-1696 |
| 牛肠激酶催化亚基工程菌培养条件的探索与鉴定 |
| Exploration and Identification of SuitableCulture Conditions for BovineEnterokinase Catalytic Subunit Engineering Bacteria |
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| DOI： |
| 中文关键词: 牛肠激酶催化亚基 工程菌 培养条件 探索 鉴定 |
| 英文关键词: Bovine enterokinase catalytic subunit Engineering bacteria Culture condition Study Identification |
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| 中文摘要: |
| 目的：探索牛肠激酶催化亚基工程菌高效表达的培养条件。方法：牛肠激酶催化亚基基因的初步表达，筛选构建工程菌，从
以下方面入手优化培养条件：培养基的组成、摇瓶发酵培养条件、培养基的PH、种龄、接种量、培养时间等，并且通过Western Blotting
和SDS-PAGE 等鉴定不同条件下的实验结果，从而确定最佳培养条件。结果：通过鉴定实验结果，从不同种平行培养条件中选择
产量最高的培养条件，作为最优培养条件。结论：成功地探索并鉴定了工程菌的适宜培养条件。 |
| 英文摘要: |
| Objective: To investigate the suitable cultivate condition of the high expression of bovine enterokinase catalytic subunit
engineering bacteria. Methods: The bovine enterokinase catalytic subunit preliminary was expressed and the engineering bacteria was
constructed. The cultivate condition was optimized from the following aspects: medium culture conditions of the optimal composition,
wave bottle fermentation cultivation condition, culture medium PH , seed age, seed number, culture time. The Western Blotting and
SDS-PAGE identification to grope the most appropriate culture condition. Results: The highest yield cultivative conditions were choice
from the same parallel culture conditions as a optimal cultivation condition. Conclusions: The culture condition of bovine enterokinase
catalytic subunit engineering bacteria had been optimized successfully. |
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