| 胡琳洁1 郭晓雅1 侯美娜1 邵成2 史皆然1.评价空肠弯曲菌外膜蛋白PEB1 介导的重组BCG 与上皮细胞
的结合能力[J].,2011,11(10):1810-1812 |
| 评价空肠弯曲菌外膜蛋白PEB1 介导的重组BCG 与上皮细胞
的结合能力 |
| Evaluation of Combination Ability Between Recombinant BCG Mediated byMembrane Protein PEB1 of C.Jejuni and Epithelium Cells |
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| DOI： |
| 中文关键词: 重组BCG 细胞黏附 HeLa 细胞 HIEC |
| 英文关键词: Recombinant BCG Adherence HeLa cell HIEC |
| 基金项目:国家自然科学基金资助（30871114） |
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| 中文摘要: |
| 目的：评价PEB1 介导的屋尘螨抗原（Der p2）重组BCG 疫苗（PEB1-Der p2- rBCG）与人上皮细胞的结合能力。方法：采用体
外细胞培养的方法，分别将普通BCG、胞壁型Der p2-rBCG 和胞壁型融合蛋白PEB1-Der p2- rBCG 与HeLa 细胞及人类肠粘膜上
皮细胞（HIEC）进行共孵育，利用HE 和抗酸染色法对各组细胞与疫苗的黏附结果进行染色，光学显微镜下计数各组的黏附率，并
进行比较；对以上各组分别加入PEB1 蛋白，进行黏附阻断，观察对结合能力的影响。结果：孵育24 小时后，无论HeLa 细胞还是
HIEC PEB1-Der p2- rBCG 组较普通BCG 组和Der p2-rBCG 组的黏附率明显提高，差异有显著性（P<0.05）；PEB1 蛋白的加入对
PEB1-Der p2- rBCG 的黏附功能有明显抑制作用（P<0.05）；但是，Der p2 -rBCG 组与普通BCG 组比较没有明显差异（P>0.05），
PEB1 蛋白的加入对二者的黏附亦无影响（P>0.05）。结论：PEB1 具有介导增强PEB1-Der p2- rBCG 与上皮细胞黏附的能力。 |
| 英文摘要: |
| Objective: To investigate the combination ability of the PEB1-Der p2- rBCG and epithelium cells. Methods: The
method of cell culture in vitro was used to culture the bacterial strains of common BCG，membrane form Der p2-rBCG and membrane
form PEB1-Der p2- rBCG with HeLa and human intestinal epithelial cells (HIEC)，HE staining and acid-fast staining were used and the
adhesion of cells was detected at light microscopy; purified PEB1was added into each group to adhesion blocking，and the influence of
combining ability was observed. Results: After incubated for 24 h，no matter HeLa cells or HIEC，adhesion rate of PEB1-Derp2- rBCG
was obviously improved than common BCG and Der p2-rBCG, and the differences were statistically significant (P < 0.05); The
differences between PEB1-Der p2- rBCG performed in the absence and presence of PEB1 protein were significant (P < 0.05); However，
the Der p2-rBCG bacteria group was not significantly different from common BCG group (P > 0.05) and no adhesion blocking effects in
the two group which added PEB1 protein (P > 0.05). Conclusion: The PEB1 increased the adhesion ability which mediated PEB1- Der p2
- rBCG and epithelium cells. |
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