| 王琰1 杨瑛2△ 刘兵3 陈宥艺1 陈冬波3.骨髓间充质干细胞移植对VCD 所致卵巢损伤的修复研究[J].,2011,11(10):1844-1850 |
| 骨髓间充质干细胞移植对VCD 所致卵巢损伤的修复研究 |
| Bone Marrow Mesenchymal Stem Cell Transplantation for PrematureOvarian Failure Induced by 4-vinylcyclohexene Diepoxide |
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| DOI： |
| 中文关键词: 骨髓间充质干细胞 VCD 卵巢早衰 模型 小鼠 |
| 英文关键词: Bone marrow mesenchymal stem cell VCD Premature ovarian failure Model Mouse |
| 基金项目:国家“863”计划资助子课题（2008AA8070099） |
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| 中文摘要: |
| 目的：探讨小鼠骨髓间充质干细胞（MSCs）移植对去氧乙烯基环己烯（VCD）所致卵巢早衰治疗的可行性。方法：采用VCD
（160mg kg-1,day-1）连续腹腔注射来诱导小鼠卵巢早衰。每侧卵巢注射转染了绿色荧光基因小鼠骨髓来源的MSCs，于移植后14、
28 天及45 天，取各组血液标本及卵巢组织，同时观察小鼠动情周期的变化；酶联免疫法检测血清FSH、LH 水平，显微镜下观察
MSC 在卵巢的分布。结果：MSCs 移植后各组均可见绿色荧光，并且主要分布于卵巢间质区，卵巢泡膜细胞区也可见绿色荧光细
胞。MSCs 组动情周期较实验对照组缩短，FSH 与LH 水平较实验对照组低，差异具有显著性。结论:骨髓间充质干细胞可改善卵巢
早衰小鼠的卵巢内分泌功能，并且长时间存在于卵巢组织。骨髓间充质干细胞可能成为卵巢早衰治疗的新方法。 |
| 英文摘要: |
| Objective: To investigate the therapeutic potency of MSCs transplantation on premature ovarian failure induced by
4-vinylcyclohexene diepoxide (VCD).Methods: Female C57 mice were dosed with VCD (160mg kg-1 day-1, ip) daily to induce ovarian
failure. GFP-labeled BMSCs were injected into the ovary. The FSH and LH in the serum were detected and the ovaries were collected at
14d, 28d and 45d after transpantatipn. GFP-labeled MSCs were tracked in the ovaries. Ovarian follicle populations were also determined.
Results: There were fluorescent cells in ovarian tissues of every time after transplantation. Estrous cycles were shorten in the
transplantation group than that in the model group. The FSH and LH in transplantation group at 14, 28, 45days after transplantation were
significantly lower than those in the model group. Conclusions: The transplantation MSCs can improve the ovary function which
damaged by VCD. MSCs can migrate in the mouse ovaries and exist in ovarian tissues of mouse for a long time. MSCs may be a new
treatment for premature ovary failure. |
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