| 马寅姣1 邹秉杰1 王建平1 周国华1，2.基于核酸侵入反应的生物分子检测技术的研究进展[J].,2011,11(12):2384-2388 |
| 基于核酸侵入反应的生物分子检测技术的研究进展 |
| Advances in Bio-molecular Diagnostic Assays Based on Invasive Reaction |
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| DOI： |
| 中文关键词: 核酸侵入反应 5' 核酸内切酶 Flap 内切酶 信号扩增 |
| 英文关键词: Invasive reaction 5' Endonuclease Flap endonuclease Signal amplification |
| 基金项目:江苏省自然科学基金(No. BK2010111) ; 国家自然科学基金项目(No. 21005088) |
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| 中文摘要: |
| 核酸侵入反应是由5' 核酸内切酶或flap 内切酶催化的，能够识别切割核酸片段形成的特异性结构的一类反应。近年来发展
了很多基于该反应的生物大分子检测技术，能够对DNA、RNA、miRNA 及蛋白质进行高灵敏、高特异性的测定。这些技术大都无
需扩增待测靶标，极大地降低了扩增产物交叉污染的风险，在临床检测中具有很大的应用前景。本文对这些检测技术的原理及应
用作简要综述。 |
| 英文摘要: |
| The invasive reaction is catalyzed by 5' endonuclease or flap endonuclease, which can recognize and cleavase a
specific structure formed by oligonucleotides. Recently, numerous newly techniques based on invasive reaction have been developed to
detect biological molecules, such as DNA, RNA, miRNA and protein, with a high sensitivity and specificity. These techniques do not
depend on target amplification, so the risk of cross-contamination decreased significantly, and it show great potential in clinical
diagnostics. This article gives a review on the principles and applications of these developed assays. |
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