| 罗四维1 汪世平2.抗日本血吸虫pVAX1/ SjHGPRT·SDISP 多价疫苗对昆明小鼠的保护
作用及其机制探讨[J].,2011,11(18):3413-3416 |
| 抗日本血吸虫pVAX1/ SjHGPRT·SDISP 多价疫苗对昆明小鼠的保护
作用及其机制探讨 |
| Immunoprotection and Mechanism Exploration of Multivalent DNAVaccine pVAX1/SjHGPRToSDISP Against Schistosoma Japonicum |
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| DOI： |
| 中文关键词: 血吸虫 多价疫苗 pVAX1/SjHGPRToSDISP 机制 |
| 英文关键词: Schistosoma japonicum Multivalent DNA vaccine pVAX1/SjHGPRToSDIS Mechanism |
| 基金项目:国家重点基础研究发展（973）计划（No. 2007CB513108）；国家科技支撑计划（No. 2009BAI78B05）；
湖南省血吸虫病免疫与传播控制重点实验室、湖南省重点学科建设专项经费（No. 07-985-2） |
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| 中文摘要: |
| 目的：探讨抗日本血吸虫生殖产卵编码基因多价疫苗pVAX1/ SjHGPRToSDISP 对昆明小鼠的保护作用及其机制。方法：选
取昆明小鼠30 只，分别使用pVAX1/ SjHGPRToSDISP、pVAX1 以及生理盐水，每只小鼠100μg 或等量经左腿股四头肌注射。处
理2 周后，采集动物模型血样检测IgG、IL-2, IL-4, IL-10 以及INF-γ表达量。处理4 周后，以20±1 条尾蚴贴腹感染，感染6 周后
检测减虫率、减卵率。结果：尾蚴攻击动物后6 周，pVAX1/SjHGPRToSDISP 免疫组的肝脏减虫率为42.2%，子宫与肝脏减卵率分
别为68.04%以及72.96%。与对照组比较，差异有显著性。pVAX1/SjHGPRToSDISP 免疫组虫体内IgG、IL-4 以及INF-γ表达量明
显升高，与对照组比较差异有显著性（P<0.05）。结论：pVAX1/SjHGPRToSDISP 多价疫苗具有较好的免疫保护作用，且该类作用
的机制与IgG、IL-4 以及INF-γ 的表达升高存在关联。 |
| 英文摘要: |
| Objective: To explore the protection of multivalent DNA vaccine pVAX1/SjHGPRToSDISP in Kunming mice and it's
mechanism. Methods: A total of 30 Kunming mice were selected and treated by pVAX1/ SjHGPRToSDISP, pVAX1 and normal saline
respectively. Each mouse was injected with 100μg or equivalent amount through quadriceps of left legs. Two weeks later after the injection,
the serum of the mice was selected to detect the expression level of IgG, IL-2, IL-4, IL-10, INF-γ. After 4 weeks of the injection,
the mice were infected by the procercoid through abdominal wall. After the infection for 6 weeks, the worm reduction rate and the egg
reduction rate were investigated. Results: The worm reduction rate of the pVAX1/SjHGPRT·SDISP was 42.2%. The egg reduction rates
of the pVAX1/SjHGPRToSDISP in uterus and liver were 68.04% and 72.96%,respectively, which were significantly different from the
control group (P<0.05). The expression level of IgG, IL-4 and INF-γ in pVAX1/SjHGPRToSDISP immune group is significantly higher
than that of the control group (P<0.05). Conclusion: The pVAX1/SjHGPRToSDISP showed good immnunoprotection, which was correlated
with the increased expression level of IgG, IL-4, INF-γ. |
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