| 张海华1 孟瑾2 张峰2△.细胞周期蛋白依赖激酶Roscovitine 诱导非小细胞肺癌A549 细胞凋亡及
其机制的研究[J].,2011,11(20):3835-3837 |
| 细胞周期蛋白依赖激酶Roscovitine 诱导非小细胞肺癌A549 细胞凋亡及
其机制的研究 |
| Cyclin Dependent Kinase Inhibitor Roscovitine-induced Apoptosis inNon-small Cell Lung Cancer A549 Cells and Its Mechanisms |
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| DOI： |
| 中文关键词: Roscovitine 肺癌细胞；细胞凋亡；Bax Bad |
| 英文关键词: Roscovitine Non-small cell lung cancer Apoptosis Bax Bad |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨细胞周期蛋白依赖激酶（CDK）抑制剂Roscovitine（Ros）诱导非小细胞肺癌（NSCLC）A549 细胞凋亡及其作用机
制。方法：以不同浓度Ros（10μM、20μM、40μM）处理细胞24h，采用Annexin V-PI 染色以流式细胞仪检测细胞凋亡，Western
blot 法检测胞浆中和线粒体促凋亡蛋白Bax 和Bad 的表达，流式细胞仪检测线粒体膜电位（MMP）变化。结果：Ros 以剂量依赖的
方式诱导A549 细胞凋亡，同时Bad 和Bax 在胞浆的含量随着Ros 剂量的增加而减少，而在线粒体中却出现相反的结果，线粒体
膜电位随Ros 剂量的增大而降低。结论：Ros 可通过促进Bax 和Bad 由胞浆向线粒体易位，诱导NSCLC A549 细胞由线粒体途径
发生凋亡。 |
| 英文摘要: |
| Objective: To investigate the effect of cyclin dependent kinase inhibitor roscovitine (Ros)-induced apoptosis on
non-small cell lung cancer A549 cells and its mechanisms. Methods: A549 cells were exposed to varying concentrations of Ros for 24h.
Apoptosis was detected with Annexin V-PI staining by flow cytometry. The expression of pro-apoptotic protein Bax and Bad in
cytoplasm and mitochondria was detected by western blot. The changes of mitochondrial membrane potential (MMP) were analyzed by
flow cytometry. Results: Roscovitine could effectively induce apoptosis and this effect increased with the increasing of the drug
concentration. The expression levels of Bax and Bad in the cytosol declined with Ros treatment, while increased in the mitochondria, and
which was in a dose-dependent manner. Ros also caused decreasing of MMP dose-dependently. Conclusions: Ros induced apoptosis in
A549 cells in a dose-dependent way by facilitating mitochondrial translocation of Bax and Bad, thus activating mitochondrial apoptotic
pathway. |
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