| 郑瑾1,2 刘强3 史恒军2 任秦有2 杨建刚2 刘新平4 刘文超.NDRG2 通过影响CD24 调控乳腺癌细胞的粘附能力[J].,2012,12(1):5-8 |
| NDRG2 通过影响CD24 调控乳腺癌细胞的粘附能力 |
| NDRG2 Expression Regulates CD24 and Adhesion Potentialof Breast Cancer Cells |
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| DOI： |
| 中文关键词: NDRG2 CD24 乳腺癌细胞 粘附 |
| 英文关键词: NDRG2 CD24 Breast cancer cell Adhesion |
| 基金项目:国家自然科学基金（81072973 |
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| 中文摘要: |
| 目的：阐明NDRG2（N-Myc downstream-regulated gene 2）在乳腺癌细胞中对CD24 的调控及其对乳腺癌细胞粘附能力的影
响。方法：RT-PCR 和Western blot 方法检测乳腺癌细胞MCF-7 及Bcap-37 中NDRG2 和CD24 的表达；通过腺病毒上调MCF-7
细胞中NDRG2 的表达，或利用siRNA 下调Bcap-37 细胞中NDRG2 的表达，检测CD24 基因和蛋白的变化。粘附实验检测改变
NDRG2 表达水平后对MCF-7 及Bcap-37 细胞粘附能力的影响。结果：MCF-7 细胞中NDRG2 基因和蛋白的表达水平低于
Bcap-37 细胞,而CD24 的表达水平高于Bcap-37 细胞；在MCF-7 细胞中通过腺病毒载体上调NDRG2 可以抑制CD24 的表达并
抑制其粘附能力，而在Bcap-37 细胞中利用siRNA 下调NDRG2 的表达可以提高CD24 的水平及细胞的粘附能力；结论：NDRG2
通过影响CD24 参与调控乳腺癌细胞的粘附能力。 |
| 英文摘要: |
| Objective: To investigate the effect of N-Myc downstream-regulated gene 2 (NDRG2) on the adhesion potential of
breast cancer cells. Methods: Gene and protein expressions of NDRG2 and CD24 were analyzed in MCF-7 and Bcap-37 cells. The expression
of CD24 and cell adhesion ability was detected after NDRG2 by adenovirus or siRNA treatment. Results: Both the gene and protein
expressions of NDRG2 in MCF-7 cells were lower than Bcap-37 cells. In contrast, CD24 expression is higher in MCF-7 cells compared
to that in Bcap-37 cells. Up-regulated NDRG2 expression leads to the decrease in CD24 expression and cell adhesion ability in
MCF-7 cells. In contrast, NDRG2 down-regulation results in enhancing the expression of CD24 and cell adhesion potential in Bcap-37
cells. Conclusion: NDRG2, associated with altered CD24 expression, regulates breast cancer cells adhesion ability. |
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