| 吴惠1 杨洁2 李占亭2 杨峰2 刘丽丽2 蒙军平2 王莉2 杜德伟2△.TGF-βII 型受体的RNA 干扰抑制肾纤维化[J].,2012,12(3):449-452 |
| TGF-βII 型受体的RNA 干扰抑制肾纤维化 |
| RNA Interference on TGF-βtypeII Receptor Inhibitedthe Renal Fibrosis |
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| DOI： |
| 中文关键词: TGF-βII 型受体 RNA 干扰 肾纤维化 |
| 英文关键词: TGF-β Type II receptor RNA interference Renal fibrosis |
| 基金项目: |
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| 中文摘要: |
| 目的：研究针对TGF-β II 型受体的RNA 干扰质粒对α-SMA 表达的抑制作用，探讨RNA 干扰TGF-β RII 的方法对肾间
质纤维化的抑制作用。方法：单侧结扎输尿管方法制备肾间质纤维化小鼠动物模型。分别经输尿管逆行注射a：RNA 干扰质粒；b：
错配对照质粒；c：空载体；d：生理盐水；e：正常对照。通过western-blot 及免疫组织化学方法检测第3、5、10 天后肾组织内TGF-β
RII、α-SMA，观察其对肾间质纤维化的抑制作用。结果：针对TGF-β RII 的RNA 干扰质粒，明显抑制肾组织内TGF-β RII、
α-SMA 表达；几组对照未见相应作用。结论：针对TGF-βRⅡ的RNA 干扰质粒，能够抑制肾间质纤维化的发生、发展，可能成为
延缓肾功能减退的有效方法。 |
| 英文摘要: |
| Objective: To investigate the inhibitory effect of RNA interference against TGF-β II receptor (TGF-β R Ⅱ) on
α-SMA expression and renal fibrosis. Methods:The Unilateral ureteral obstruction (UUO) model which was made by using an established
procedure was performed for the injected mice to evaluate the anti-fibrotic effect. The formulation includes: pSUPER-TGF-β RII
（100μg/mouse）, mismatch pSUPER-TGF-β RII （100μg/mouse）, empty pSUPER （100μg/mouse）, saline , nothing. The inhibition
effect of the siRNA about TGF-β RII on renal fibrosis were detected. TGF-β RII and α-SMA were detected by western-blot and imm -
unol histochemistry at 3d, 5d and 10d after the injection. Results:The injection of plasmid DNA of pSUPER-TGF-β RII decreased
significantly the level of TGF-β RII, α-smooth muscle actin (a-SMA) and collagen type I over-expression incontrast to free plasmid
DNA injection. Conclusion: Retrograde injection of TGF-β R siRNA expression vector plasmid DNA is available to suppress progressi -
on of renal interstitial fibrosis. |
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