| 佟铸谷涌泉△ 李建新张淑文李学锋郭连瑞郭建明汪忠镐张建.骨髓间充质干细胞和内皮祖细胞移植促进血流重建的比较[J].,2012,12(4):623-625 |
| 骨髓间充质干细胞和内皮祖细胞移植促进血流重建的比较 |
| The Comparison of Revascularization after Bone Morrow Mesenchymal StemCells and Endothelial Progenitor Cells Transplantation |
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| DOI： |
| 中文关键词: 间充质干细胞 内皮祖细胞 细胞移植 肢体缺血 |
| 英文关键词: Mesenchymal stemcells Endothelial progenitor cells Cell transplantation Limb ischemia |
| 基金项目:首都医科大学基础- 临床科研合作基金(10JL23) |
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| 中文摘要: |
| 目的：比较骨髓间充质细胞(Bone Marrow Mesenchymal Stem Cells, BM /MSC)和骨髓源内皮祖细胞(Bone Marrow Endothelial
progenitor cells, BM /EPC) 移植促进血流重建的效果，为进一步优化骨髓干细胞移植治疗肢体缺血提供理论基础。方法：获取
Lewis 大鼠骨髓单个核细胞，在体外培养分化为MSC 和EPC。采用Lewis 大鼠建立单侧后肢缺血模型。在模型建立后3 天，将
0.8mlD-Hanks 液注入大鼠缺血侧后肢，为对照组（n=6）；将8×106 个骨髓MSC 植入大鼠缺血侧后肢，为MSC 组（n=6）；将体外培
养的8×106 个EPC 植入大鼠缺血侧后肢，为EPC 组（n=6）。细胞移植后3 周行缺血大鼠后肢动脉造影，检测缺血侧后肢侧支血管
数；获取缺血侧后肢腓肠肌，分别行CD31 和α-SMA 免疫组化染色，计算毛细血管密度和小动脉密度。结果：MSC 组与EPC 组侧
支血管数无显著性差异，二者均高于对照组；EPC 组毛细血管密度明显高于MSC 组，二者均高于对照组；MSC 组与EPC 组小动
脉密度无显著性差异，二者均高于对照组。结论：骨髓间充质干细胞移植和内皮祖细胞移植均能够明显促进血流重建，而且骨髓
间充质干细胞在治疗肢体缺血性疾病中的优势应该受到重视。 |
| 英文摘要: |
| Objective: To compared the revascularization after bone marrow mesenchymal stem cells(BM/MSC) and bone marrow
endothelial progenitor cells （BM/EPC）transplantation and investigate the roles of different stem cells in revascularization. Methods:
MNC were harvested from Lewis rats bone marrow. The MSC and EPC were obtained after MNC were cultured for transplantation.
Lower limb ischemic model was made with Lewis rats. After limb ischemic model were made, 0.8ml D-Hanks , 8×106MSCs and 8×106
EPCs were injected into control group (n=6), MSC group (n=6) and EPC group (n=6) respectively. At 3 weeks after D-hanks
injection/cells transplantation, the quantity of collateral was observed with digital subtraction angiography (DSA). Meanwhile, the
CD31and α-SMA of the gastrocnemius muscle obtained from the ischemic limb were tested with immunohistochemical stain. Results:
Compared with MSC group, the density of the capillaries in EPC group was significantly higher, and there were significant differences
between control group and treatment groups. There was no significant difference between MSC group and EPC group in the number of
collaterals and density of arterioles. Conclusion: Both MSC and EPC transplantation can improve revascularization significantly. The
roles of revascularization of MSC should be attached importance to in the stem cells transplantation to treat for limb ischemic disease. |
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