| 吴利英王明义辛晓燕△ 陈必良.人卵巢癌A2780 细胞侵袭转移与HES1 相关性[J].,2012,12(5):829-832 |
| 人卵巢癌A2780 细胞侵袭转移与HES1 相关性 |
| The Study of Relationship between HES1 and Invasion and MetastasisPotency of Human Ovary Cancer Cell A2780 |
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| DOI： |
| 中文关键词: 卵巢癌 DAPT HES1 侵袭 转移 |
| 英文关键词: Ovary cancer DAPT HES1 Invasion Metastasis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨人卵巢癌细胞A2780 中的HES1 表达与人卵巢癌侵袭及转移的关系。方法：分别运用实时RT-PCR、Western
Blotting 及细胞免疫荧光法检测γ 分泌酶抑制剂作用前后A2780 细胞中HES1 的表达水平，Transwell 小室试验及MTT 法分别用
于检测细胞的侵袭、迁移及黏附能力。结果：下调A2780 细胞中HES1 的表达水平后，A2780 细胞的黏附、侵袭及转移能力下降，
差异有统计学意义（P＜0. 01）。结论：HES1 的表达与人卵巢癌细胞的侵袭及转移密切相关，下调其表达可抑制卵巢癌细胞的黏
附、侵袭及转移能力。 |
| 英文摘要: |
| Objective: To investigate the relationship between HES1 and the invasion and metastasis ability of A2780 cells by
down-regulating its expression. Methods: The RNA level of HES1 was detected by real-time RT-PCR (reverse transcription polymerase
chain reaction) method. Western Blotting was used to detect the protein level of HES1.The adhesion assay was used to detect the cancer
cell adhesion abilities, the invasion and migration abilities were detected by Transwell chambers. Results: After down-regulating the
expression of HES1, the adhesion , invasion and migration abilities of A2780 cells decreased（P＜0. 01）.
Conclusion: The expression of
HES1 may be correlated with invasion and metastasis potency of hunman ovary cancer and downregulating its expression can inhibit the
adhesion, invasion and migration abilities. |
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