| 徐菲菲1 于小玲1△ 尚芳芳2 赵辉2 李红静1.去甲斑蝥素对H446 细胞Id1mRNA 表达的影响[J].,2012,12(7):1225-1228 |
| 去甲斑蝥素对H446 细胞Id1mRNA 表达的影响 |
| Norcantharidin Inhibits the Expression of Id1 mRNA in H446 Cells |
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| DOI： |
| 中文关键词: 去甲斑蝥素 Id1 H446 细胞 |
| 英文关键词: Norcantharidin Id1 H446 cells |
| 基金项目: |
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| 中文摘要: |
| 目的：观察去甲斑蝥素对小细胞肺癌H446 细胞Id1mRNA 表达的影响。方法：分别利用MTT 法检测细胞生长活性；用划痕
实验分析细胞迁移能力；采用Hoechst 染色观察细胞凋亡；用实时荧光RT-PCR 法测定H446 中Id1mRNA 的表达。结果：去甲斑
蝥素对H446 细胞的生长有明显的抑制作用，细胞的生长抑制率和凋亡率明显增加，细胞迁移距离明显缩短。去甲斑蝥素可抑制
细胞内Id1mRNA 的表达，其相对定量随去甲斑蝥素的浓度增大而减少。结论：在H446 细胞中，去甲斑蝥素能抑制Id1mRNA 的
表达，这可能是去甲斑蝥素抑制细胞生长，迁移和诱导细胞凋亡的重要机制之一。 |
| 英文摘要: |
| Objective: To investigate the effects of the expression of Id1mRNA on human small cell lung cancer H446 cells.
Methods: The cell viability of H446 was evaluated by MMT assay. The ability of cell migration was determined by scratch wound assay.
The cell apoptosis was assayed by Hoechst staining. The quantity of Id1 mRNA of H446 cells was detected by real time quantitative
reverse transcription-polymerase chain reaction (RT-PCR). Results: The cell inhibition rate and cell apoptosis rate increased obviously by
norcantharidin. The cell migrating distance was shorter in norcantharidin treated cell. Meanwhile, the relative quantity of Id1mRNA
reduced significantly by norcantharidin. The quantity of Id1mRNA was inhibited by norcantharidin in a dose dependent manner.
Conclusion: Norcantharidin can inhibit Id1mRNA in H446 cells, which may be one important mechanism of decreased cell viability,
migration ability and induced cell apoptosis. |
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