| 蔡越於进文白云刚刘焕王忠超暴军香马进△.间断性人工重力对模拟失重大鼠颈总动脉平滑肌细胞凋亡的影响[J].,2012,12(10):1820-1823 |
| 间断性人工重力对模拟失重大鼠颈总动脉平滑肌细胞凋亡的影响 |
| Effects of Intermittent Artificial Gravity on Apoptosis of Common CarotidSmooth Muscle Cells in Simulated Microgravity Rats |
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| DOI： |
| 中文关键词: 微重力 颈总动脉 血管平滑肌细胞 凋亡 大鼠 |
| 英文关键词: Simulated microgravity Carotid Smooth muscle cells Apoptosis Rat |
| 基金项目:国家自然科学基金项目(30871218 ) |
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| 中文摘要: |
| 目的：研究3 周模拟失重大鼠颈总动脉平滑肌细胞凋亡的变化及间断性人工重力对其的影响。方法：以尾部悬吊大鼠(SUS)
模拟失重，同期每天悬吊23h、站立1h(STD)模拟间断性人工重力的对抗效果，用M30 染色及Tunel 染色方法观察3 周SUS 组、
同步对照(CON)组及STD 组颈总动脉平滑肌细胞早期和中晚期的凋亡情况，并用免疫组织化学方法及Western blot 印迹方法观
察各组大鼠颈总动脉组织Caspase-3 的蛋白表达变化。结果：与CON 组比较，SUS 组大鼠颈总动脉平滑肌细胞M30 染色阳性细
胞明显减少, STD 组M30 染色阳性细胞较CON 组及SUS 组显著增加；SUS 组Tunel 染色阳性细胞较CON 组及STD 组显著减
少，STD 组Tunel 染色阳性细胞较CON 组及SUS 组显著增加；SUS 组Caspase-3 的表达较CON 组显著降低（P<0.05)，STD 组
Caspase-3 的表达较CON 组及SUS 组显著增高（P<0.01)。结论：模拟失重可引起大鼠颈总动脉平滑肌细胞凋亡减少，每日1 h 的
-Gx 对抗使颈总动脉的凋亡增加。Caspase-3 可能在调控模拟失重所致血管组织平滑肌细胞的凋亡中发挥作用。 |
| 英文摘要: |
| Objective: To investigate the effects of 3 weeks simulated microgravity and intermittent artificial gravity on apoptosis
of common carotid smooth muscle cells (CCSMCs) in rats. Methods: Tail-suspension rats (SUS) were used to simulate the effects of
microgravity, and homochronous countermeasure standing (STD, daily 1 h of -Gx gravitation) was used to simulate the effects of
intermittent artificial gravity (IAG) .The apoptosis of CCSMCs was assessed by the basis of M30 CytoDEATH Fluorescein（M30）and
terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (Tunel) staining. The protein expression of Caspase-3 in
common carotid was demonstrated by Immunohistochemistry and Western blot. Results: After 3-week simulated microgravity and
intermittent artificial gravity, compared with CON , M30 and TUNEL positive CCSMCs in SUS rats decreased significantly, while M30
and TUNEL positive CCSMCs increased significantly in STD; Compared with SUS, M30 and TUNEL positive CCSMCs in STD rats
increased significantly . Compared with CON and STD , the expressions of Caspase-3 decreased significantly (P < 0.01 respectively) in
SUS , and compared with CON and SUS, the expression of Caspase-3 increased significantly (P < 0.01 respectively) in STD.
Conclusions: The decrease of apoptosis CCSMCs can be induced by simulated microgravity, while intermittent artificial gravity can
cause the increase of apoptosis CCSMCs. These findings further suggest that the expression of Caspase-3 may mediate the alteration in
apoptosis of CCSMCs induced by microgravity or intermittent artificial gravity (IAG) . |
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