| 陈清华王文岚任杰李娅谢小萍凌孝臣李金声△.常压氧与高压氧对成年大鼠脑缺血再灌注损伤后
微血管新生影响的比较[J].,2012,12(10):1872-1875 |
| 常压氧与高压氧对成年大鼠脑缺血再灌注损伤后
微血管新生影响的比较 |
| Comparison of Normobaric Oxygen and Hyperbaric Oxygen on Angiogenesisin the Brain of Adult Rats with Cerebral Ischemia-Reperfusion Injury |
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| DOI： |
| 中文关键词: 高压氧 脑缺血 再灌注损伤 中动脉栓塞 血管新生 |
| 英文关键词: HBO Cerebral ischemia Reperfusion injury Middle cerebral artery occlusion Angiogenesis |
| 基金项目:国家自然科学基金（81101466），陕西省科学技术研究发展计划项目（2011K14-07-12） |
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| 中文摘要: |
| 目的：研究常压氧与高压氧对成年大鼠脑缺血再灌注损伤后微血管新生影响的差异。方法：将成年SD 雄性大鼠随机分为
三组：假手术组（SS 组）、常压氧治疗组（NBO 组）、高压氧治疗组（HBO 组），每组又随机分为3、7、10 天三个亚组。采用线栓法对
NBO 组和HBO 组大鼠进行大脑中动脉栓塞（MCAO），缺血1.5 小时后拔出栓子再灌注，NBO 组进行常压氧治疗，HBO 组进行高
压氧治疗。大鼠分别在3、7、10 天麻醉处死，取脑组织切片，血管内皮生长因子（VEGF）、VEGF 受体-1（FLT-1）和CD34 免疫组化
染色，光镜观察取图和统计分析。结果：NBO 各组与SS 各组相比，VEGF、FLT-1 和CD34 阳性细胞数目均明显增多（P＜0.05）；
HBO 各组与NBO 各组比较，7 天、10 天组VEGF、FLT-1 和CD34 阳性细胞数目均显著增多（P＜0.05）。结论：HBO 治疗较NBO
治疗对成年大鼠微血管的新生更有促进作用。 |
| 英文摘要: |
| Objective: To investigate the difference between normobaric oxygen (NBO) and hyperbaric oxygen (HBO) on
angiogenesis in the brain of adult rats with cerebral ischemia-reperfusion injury. Methods: Adult male Sprague-Dawley (SD) rats were
randomly divided into three groups: the sham surgery (SS) group, the NBO group and the HBO group. Each group was randomly divided
into three subgroups: the 3 days group, the 7 days group and the 10 days group. Middle cerebral artery occlusion (MCAO) was performed
to the NBO and HBO groups to develop the animal model. 1.5 hours later, reperfusion was made. Then, the NBO group received NBO
treatment, and the HBO group received HBO treatment. All the rats were sacrificed respectively on day 3, 7, 10; Cerebral samples were
collected and prepared to sections. The sections were treated with immunohistochemical staining with the following antibody: Vascular
endothelial growth factor (VEGF), VEGF receptor-1 (Flt-1), CD34. The changes in ischemia region were detected under the optical
microscope , and the pictures were taken. Results: Compared with those in the SS group, numbers of VEGF and Flt-1 and CD34 positive
cells increased markedly in the NBO group (P＜0.05). The numbers of VEGF and Flt-1 and CD34 positive cells increased significantly
on day 7 and day 10, following HBO treatment compared with those in the NBO group (P＜0.05). Conclusions: HBO therapy is better to
promote angiogenesis in the brain of adult rats with ischemia-reperfusion injury than NBO treatment. |
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