| 姜利军1 周晓曦3 于飞2 黄亮3 马全富2 周剑锋1 曹阳1△.FLT4 基因在斑马鱼早期发育过程中的表达[J].,2012,12(12):2224-2226 |
| FLT4 基因在斑马鱼早期发育过程中的表达 |
| The Expression of FLT4 (VEGFR3) Gene during the Early Stageof Development in Zebrafish* |
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| DOI： |
| 中文关键词: 斑马鱼 FLT4 原位杂交 |
| 英文关键词: Zebrafish FLT4 Hybridization in situ |
| 基金项目:国家自然科学基金青年基金(81001049) |
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| 中文摘要: |
| 目的：研究FLT4（VEGFR3）基因在斑马鱼早期发育过程中的表达。方法：提取斑马鱼胚胎的总RNA，制备地高辛标记的
FLT4RNA 反义探针，WISH（整胚胎原位杂交）研究FLT4在斑马鱼早期发育过程中的表达。结果：成功合成FLT4 基因
探针，获得FLT4 基因在斑马鱼早期发育过程中的表达情况：FlT4 在2-cell、32-cell、oblong、shield 期前普遍性表达( 0.75h、1.7h、
3.7h、6h ) ；24h 在头部表达较多，特别是在ICM（intermediate cell mass，中间细胞群）区处有特异性表达；48h、72h 在头部表达均较
高表达。结论：FLT4 在早期参与了血管的形成和造血的发生，在脑部和肾小管的发育中可能起到了重要作用。 |
| 英文摘要: |
| Objective: To study the expression of FLT4 (VEGFR3) gene during the early stage of development in zebrafish.
Methods: The total RNA of different stages of zebrafish embryos was extracted, digoxigenin-labeled antisense RNA probe of FLT4 was
prepared, WISH (whole embryo mount in situ hybridization) was carried out to investigate the expression pattern of FLT4 in early stage
of development in zebrafish. Results: We synthesized gene probe for FLT4 successfully, and obtained the pictures and data of early
expression of FLT4 gene in zebrafish: FLT4 transcript was distributed ubiquitously before the shield stage ( during 2-cell stage to shield
stage); At 24 hour after post fertilization(hpf), there were obvious expression in the brain,especially in the ICM ( intermediate cell mass)
region; AT 48hpf and 72hpf, there were detectable expression in the brain. Conclusion: The expression of FLT4 during the early stage of
zebrafish suggests that it may be involved in angiogenesis and hematopoiesis, and it may be critical for the development of the brain and
renal tubule. |
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