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目的：研究凋亡素2 配体(Apo-2 ligand，Apo-2L)，或称肿瘤坏死因子相关凋亡诱导配体（TNF-related apoptosis-inducing ligand， TRAIL) 在体外对人肺腺癌A549 细胞系的放射增敏作用的研究。方法：MTT 法检测Apo-2L 单药或与放射线联合对腺癌 A549 细胞的抑制率，将细胞分为4 组，对照组、Apo-2L 组、Apo-2L+ 放射照射组、单纯照射组，200ng/ml、286 ng/ml 的Apo-2L 作用24 小时后给予放射照射，照射剂量分别为：(1Gy、1.4 Gy、1.8Gy、2 Gy、3 Gy),然后进行流式细胞仪分析照射后24h 各组细胞凋亡率变化。结果：MTT 结果显示，腺癌A549 细胞的抑制率与Apo-2L 的浓度成正相关，凋亡素2 配体作用24h 后IC50 为286 ng／ml.流式细胞仪分析显示286ng／ml 的Apo-2L 处理24h 后，细胞凋亡率从（6.68）%上升至（50）%，照射后24h Apo-2L+ 照射组凋亡率明显升高，为72.790%，对照组0.1185%，Apo-2L 组50%，单纯照射组51.5067%。结论：Apo-2L 在体外对腺癌A549 细胞有抑制增殖和促进凋亡作用，并且Apo-2L 联合放射线可以明显提高腺癌A549 细胞的凋亡率。

英文摘要:

Objective: To investigate the effect of Apoptosis-2 ligand (Apo-2L) which was also known as Tumor Necrosis Factor- related apoptosis-inducing ligand (TRAIL) on lung adenocarcinima A549 cell lines. Methods: All adenocarcinima A549 cells were randomly divided into four groups: group reaccepted Apo-2L, group reaccepted both Apo-2L and radiation, group reaccepted radiation alone and control group. The two of them were prescribed with different levels of Apo-2L (200ng/mL, 286ng/mL). After 24 hours, three groups were irradiated in different dose level (1Gy, 1.4Gy, 1.8Gy, 2.0Gy and 3.0Gy). The apoptosis rates of all groups were analyzed by Flow cytometry 24 hours later. The inhibition rates of the A549 cell lines were measured by the yellow tetrazolium (3-(4, 5-dimethylthiazolyl- 2)-2, 5-diphenylterazolium bromide) MTT. Results: The analysis of Flow cytometry showed that the rates of cells apoptosis were escalated from 6.68% to 50% after IC50 for 24 hours. The results of MTT showed that the correlation between the inhibition rate and the concentration of Apo-2L in adenocarcinoma A549 cells is positive. The apoptosis rates of the four groups were 72.8%, 0.12%,50%, 51.5%. Conclusion: The Apo-2L can inhibit the proliferation and promote the apoptosis of adenocarcinoma A549 cells in vitro and improve the apoptosis of adenocarcinoma A549 cells significantly if combined with radiation.

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