| 王冬梅戴飞红洪炎国周小龙阮丽钦.慢性激活MrgC 受体上调CGRP表达的nNOS机制*[J].,2014,14(2):214-216 |
| 慢性激活MrgC 受体上调CGRP表达的nNOS机制* |
| The Mechanismof Up-Regulation of CGRP on MrgC in Cultured DorsalRoot Ganglia of Rats* |
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| DOI： |
| 中文关键词: MrgC 受体（SNSR） 背根神经节 降钙素基因相关肽 一氧化氮合酶 |
| 英文关键词: MrgC receptor (Sensory neuron-specific receptors) Dorsal root ganglin Calcitonin gene-related peptide Nitric oxide
synthase |
| 基金项目:国家自然科学基金项目（30970985） |
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| 中文摘要: |
| 摘要目的：检查持续应用BAM8-22 对体外组织培养感觉神经节合成钙调素基因相关肽(CGRP)的影响。方法：将体外培养的大鼠
三叉神经节和背根神经节经BAM8-22 和L-NAME 处理后，用酶联免疫法测定CGRP 的表达含量变化。结果：与对照组相比，连
续4 天给予SNSR 的选择性激动剂BAM8-22，CGRP 的合成会增加。联合给予BAM8-22 和NOS 的非选择性抑制剂L-NAME，
CGRP的表达随不同剂量的L-NAME 引起不同程度的上调。结论：持续激活SNSR 能使感觉神经节合成CGRP增多，是在体动物
慢性激活SNSR 后吗啡镇痛作用降低的细胞学机制。 |
| 英文摘要: |
| ABSTRACT Objective：To investigate the effect of chronic BAM8-22 treatment on the expression of calmodulin gene related with
peptide ( CGRP ) in cultured sensory ganglion. Methods：DRG and TG tissue of rats were cultured in vitro after BAM8-22 and L-NAME
treatment. The changes of CGRP content was detected by enzyme linked immunosorbent assay. Results：Compared with the control
group, the expression of CGRP increased after the consecutive treatment of selective SNSR agonist (BAM8-22) for 4 days. Combined
that treated with L-NAME (a non-selective nitricoxide synthesis inhibitor), the expression of CGRP was up-regulated differently with
different doses of L-NAME. Conclution：Chronic activation of SNSR resulted in the enhanced expression of CGRP, it may be the cellular
mechanismfor reducing the analgesic effect of morphine after continuous activation of SNSR. |
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