| 黄秋琴肖奇明△ 胡成平.罗格列酮和强的松对博来霉素诱导大鼠肺纤维化中FIZZ1表达的影响[J].,2014,14(2):217-222 |
| 罗格列酮和强的松对博来霉素诱导大鼠肺纤维化中FIZZ1表达的影响 |
| The Effects of Rosiglitazone and Prednisone on the Expression of FIZZ1 inthe Pulmonary Fibrosis Induced by Blemycin |
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| DOI： |
| 中文关键词: 肺纤维化 强的松 罗格列酮 过氧化物酶体增殖物激活受体酌 发现于炎症区域 |
| 英文关键词: Pulmonary fibrosis Prednisone Rosiglitazone PPARγ Fizz1 |
| 基金项目: |
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| 中文摘要: |
| 摘要目的：观察Fizz1 在肺组织中的动态表达及其意义，并比较罗格列酮与强的松对Fizz1 表达的影响。方法：90 只雄性SD 大
鼠随机分为对照组，模型组，强的松干预组，罗格列酮干预组，强的松+ 罗格列酮干预组，模型组和干预组大鼠气管内一次性注入
博来霉素构建大鼠肺纤维化模型，对照组给予等量生理盐水。造模24 小时后各干预组给予相应药物灌胃。各组动物于7、14、28d
随机处死6 只，测肺系数,取肺组织病理切片行HE 和Masson 染色，观察肺泡炎和纤维化程度，测肺组织羟脯氨酸含量，免疫组化
法检测Fizz1在肺组织中的表达情况。结果：干预组各时期肺纤维化程度均较模型组轻。Fizz1 表达呈动态性变化，第7 天表达最
高，14 d，28 d 表达减弱。干预组各时期Fizz1 表达水平较模型组降低，其中罗格列酮和强的松联合干预组早期对Fizz 的下调水平
更大。结论：Fizz1 的表达上调可能参与博来霉素诱导的肺纤维化形成；罗格列酮、强的松均可下调Fizz1表达减轻博来霉素诱导
的肺纤维化程度，减少肺组织胶原的沉积，提示Fizz1可成为肺纤维化治疗的新靶点，罗格列酮和强的松联用治疗较单用强的松
效果更好，对Fizz1 的下调水平更大。 |
| 英文摘要: |
| ABSTRACT Objective：To estimate the effects of rosiglitazone, a PPAR-γ agonist, and prednisone on the dynamic expression of
Fizz1 in pulmonary fibrosis induced by bleomycin (BLM) in rats. Methods：Ninety adult male Sprague-Dawley (SD) rats were randomly
divided into five groups: the normal group (group N), bleomycin group (group B), the prednisone group (group P), rosiglitazone group
(group R), prednisone and rosiglitazone group (group P+R). Each group contained 18 rats. And the animals in each group at 7th, 14th,
28th days after modeling were randomly divided into 3 subgroups, each subgroup includes 6 rats. The BPF model was established by a
single dosage of 5 mg/kg bleomycin intratracheal, however group N was given the same dose of saline (NS) solution. After intratracheally
instillation of BLMfor 24h, the rats in each group received NS, prednisone, rosiglitazone or both prednisone and rosiglitazone by gavage
per day. At 7th, 14th, 28th days after BLMadministration, rats in per treatment group were randomly killed respectively. The lungs were
harvested for lung index test, hemotoxylin and eosin stain, Masson's trichorome stain and determination of hydroxyproline content. The
immunohistochemical method was used to detect the expression of fizz1. Results：The degree of fibrosis in treatment group was lower
than that in bleomycin group at every stage. Fizz1 expression had a dynamic changes. The expression of fizz1 protein in the lung
increased after 7th days in bleomycin-trected animals, and slightly decreased at 14th days, and markedly decreased at 28th days. The
expression of Fizz1 in treatment group was lower than that in model group at all stage, the expression of fizz1 in prednisone and
rosiglitazone groups were downregulated more than those in other treatment groups. Conclusion：The upregulation of the expression of
fizz1 may participate in pulmonary fibrosis induced by bleomycin. Both prednisone and Rosiglitazone can downregulate the expression of
fizz1, diminish the extent of pulmonary fibrosis induced by bleomycin, and decease the accumulation of extracellular matrix protein,
suggesting that fizz1 can become a new target in the treatment of pulmonary fibrosis. |
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