| 徐夏红陈静李俐王萌史子啸高殿帅△.GDNF的生物学效应对脂筏中胞膜蛋白定位的重要性研究*[J].,2014,14(8):1435-1437 |
| GDNF的生物学效应对脂筏中胞膜蛋白定位的重要性研究* |
| The Importance of Location of Membrane Proteins in Lipid Rafts forthe Biological Effect of GDNF* |
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| DOI： |
| 中文关键词: 脂筏 GDNF 胞膜蛋白 |
| 英文关键词: Lipid rafts GDNF Membrane protein |
| 基金项目:江苏省生物学优势学科基金（31040035/C090202）；江苏省高校学术优先发展项目（PAPD） |
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| 中文摘要: |
| 摘要目的：探讨GDNF的生物学效应对胞膜蛋白在脂筏的定位的影响。方法：首先以PBS 或GDNF 预处理体外培养的真核细
胞，提取脂筏，以免疫印迹方法检测三种胞膜蛋白（RET，NCAM140 及integrinβ1）在脂筏的含量变化。结果：GDNF预处理组RET
和NCAM140蛋白在脂筏的含量增加，而integrinβ1 蛋白的含量无显著性变化。在脂筏中也可检测到integrinβ1 蛋白。结论：
GDNF 可影响某些胞膜蛋白在细胞膜上的定位，使其招募到脂筏，这可能是GDNF的一种重要生物学效应。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the biological effect of GDNF on membrane proteins' location in lipid rafts. Three kinds of
membrane protein (RET, NCAMand integrinβ1) were taken as the objects in this study.Methods: Firstly, lipid rafts were extracted from
the cell membranes by pretreating with PBS or GDNF. Then these proteins were detected by the method of immunoblotting.
Results:Immunoblotting results showed that, the amount of RET and NCAM140 increased in lipid rafts in the presence of GDNF, while
integrinβ1 did not increase. Moreover, integrinβ1 existed in lipid rafts. Conclusiong:GDNF has effect on the location of some membrane
proteins in cell membrane and makes themmove to lipid rafts, which is perhaps a part of the biological mechanismof GDNF. |
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