| 杨菲 张统一 白录军 徐春华 方相春.人参皂甙Rd通过调节SNI大鼠背根神经节钠、钾电流抑制痛敏[J].,2014,14(12):2214-2218 |
| 人参皂甙Rd通过调节SNI大鼠背根神经节钠、钾电流抑制痛敏 |
| Ginsenoside Rd Inhibits Mechanical Pain Hypersensitivity ThroughModulating the Voltage-gated Sodium and Potassium Current in DRGNeurons in Rats of Spared Sciatic Nerve Injury |
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| DOI： |
| 中文关键词: 人参皂甙Rd 机械性痛敏 钠电流 钾电流 |
| 英文关键词: Ginsenoside Rd Mechanical pain hypersensitivity Sodiumcurrent Potassiumcurrent |
| 基金项目:国家自然科学基金项目（81070899） |
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| 中文摘要: |
| 目的：观察人参皂甙Rd（ginsenoside Rd）对大鼠坐骨神经分支选择性损伤（spared sciatic nerve injury, SNI）引起的痛敏的影
响及其作用机制。方法：坐骨神经分支选择性损伤术后7 天，观察腹腔注射不同浓度人参皂甙Rd 后大鼠后足的机械性缩足反应
阈值（paw withdrawl mechanical threshold, PWMT）的变化；在术后7 天，急性分离并取出大鼠腰4 和腰5 段背根节，对整节DRG
上的中小型神经元运用全细胞膜片钳技术进行记录。结果：坐骨神经分支选择性损伤术后7 天，大鼠出现明显的机械性痛敏，腹
腔注射5 mg/ml和10 mg/ml的人参皂甙Rd 能剂量依赖性的翻转大鼠机械性痛敏；坐骨神经分支选择性损伤能明显地增大SNI
大鼠DRG中小型神经元上的钠电流以及减小电压依赖性钾电流，而100 uM 人参皂甙Rd 能有效翻转该钠、钾电流的变化。结
论：人参皂甙Rd 能有效地改善坐骨神经分支选择性损伤引起的机械性痛敏，其机制可能与人参皂甙Rd 明显地调节SNI 大鼠
DRG中小型神经元上的电压依赖性钠、钾电流有关。 |
| 英文摘要: |
| Objective:To evaluate the effect of ginsenoside Rd on the mechanical pain hypersensitivity in rats with spared sciatic
nerve injury and probe the potential underlying mechanism.Methods:7 day post SNI, the paw withdrawl mechanical threshold was
measured before and after intraperitoneal administration of ginsenoside Rd in rats with mechanical pain hypersentivity; the L4 and L5
DRG were isolated acutely, and whole-cell recording patch clamp were performed on small- and medium-sized DRG neurons.Results:Ginsenoside Rd could dose-dependently reduce mechanical pain hypersentivity induced by spared sciatic nerve injury;
electrophysiological recordings showed that ginsenoside Rd could decrease the voltage-gated sodium current and increase the
voltage-gated potassium current in small- and medium-sized DRG neurons in rats with SNI.Conclusion:Ginsenoside Rd could reversed
the mechanical pain hypersensitivity in rats with SNI, and the potential mechanism might relate to the modulating effect of ginsenoside
Rd on the voltage-gated sodiumcurrent and the voltage-gated potassiumcurrent. |
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