| 龚伟玲1 荆凡波2 曹玉2 高雯1 徐文2△ 孙向红2△.高效液相色谱法测定大鼠血浆中来那度胺的浓度及其药动学*
龚[J].,2014,14(20):3810-3814 |
| 高效液相色谱法测定大鼠血浆中来那度胺的浓度及其药动学*
龚 |
| Study on HPLC Determination and Pharmacokinetics ofLenalidomide in Rat Plasma* |
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| DOI： |
| 中文关键词: 高效液相色谱法 来那度胺 血药浓度 药物动力学 |
| 英文关键词: HPLC Lenalidomide Plasma concentration Pharmacokinetics |
| 基金项目:山东省自然科学基金项目（ZR2011HR032） |
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| 中文摘要: |
| 摘要目的：建立高效液相色谱法测定大鼠血浆中来那度胺的浓度。方法：色谱柱采用Venusil ASB C18 column (4.6 mm× 250 mm,
5 μm) 购自于博纳艾杰尔科技有限公司。样品采用梯度洗脱，流动相为乙腈和0.05%甲酸溶液，流速为1.0 mL/min，检测波长为
254 nm，以沙利度胺为内标。结果：来那度胺血药浓度的线性范围为0.1-5 滋g/mL，最低检测限为0.1 μg/mL，三个浓度的QC 样品
(0.2, 1 and 5μg/mL)的提取回收率分别为78.8± 3.8, 80.1 ± 3.2 and 79.1 ± 7.6 %。结论：此方法准确、简便、灵敏度高，对来那度胺
的血药浓度测定和药物代谢动力学研究极具价值。 |
| 英文摘要: |
| ABSTRACT Objective:To set up a HPLC method for the determination of lenalidomide in rat plasma.Methods: The analytical
column was a Venusil ASB C18 column (4.6 mm× 250 mm, 5 μm) from Agela Technologies. Separation was achieved by gradient
elution using acetonitrile-water containing 0.05 % formic acid, with the flow rate of 1 mL/min. The UV detection wavelength was 254
nm. Thalidomide was used as the internal standard. Results:The lower limit of quantification (LLOQ) of the method was 0.1 滋g/mL and
the linear range was 0.1-5.0 μg/mL. The extraction recovery of lenalidomide was 78.8 ± 3.8, 80.1 ± 3.2 and 79.1 ± 7.6 % at
concentrations of 0.2, 1.0 and 5.0 μg/mL (QC samples), respectively.Conclusion: The method is accurate and simple with the high
sensitivity and will be valuable for the determination of lenalidomide plasma concentrations and the pharmacokinetic study. |
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