| 姜岩 张剑 王强.三种猪源性细胞外基质体内降解规律的实验研究[J].,2014,14(23):4430-4433 |
| 三种猪源性细胞外基质体内降解规律的实验研究 |
| An in Vivo Study of the Degradative Regularity about Three Porcine-derived Extracellular Matrix |
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| DOI： |
| 中文关键词: 细胞外基质 降解 弹性纤维 葡糖氨基聚糖类 |
| 英文关键词: ECM Degradation Elastin GAGs |
| 基金项目:国家自然科学基金项目（81170338）；全军医药卫生基金科技攻关项目（08G070）；
军队临床高新技术重大专项（2010gxjs060）；上海市自然科学基金项目（12ZR1449800） |
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| 中文摘要: |
| 目的：研究本实验以小鼠腹壁缺损为模型，通过对三种猪源性细胞外基质（ECM）—猪脱细胞小肠粘膜下层(P-SIS)、猪脱细
胞心包(P-PC) 以及猪脱细胞真皮（P-ADM）, 进行体内弹性纤维和葡糖氨基聚糖类(GAGs)的含量测定，然后通过三组材料中弹性
纤维和GAGs 在术后第4 周、第8 周时再生或降解的数量，来对比研究三种生物材料植入体内后的降解规律，从而为寻找性能优
良的生物补片提供理论依据，为生物补片的临床应用以及避免术后并发症方面提供指导方向。方法：BALB/c 小鼠60 只，体重18
~20 g，手术造成1 cm× 1 cm 的腹壁全层缺损，随机分为3 组（n=20），分别以等面积的P-ADM、P-PC以及P-SIS进行修复，于术
后4 周、8 周进行取材评估。结果：术后P-SIS 组的腹腔内粘连评分均低于同时期内的其余两组。术后4、8 周比较：P-ADM组，
GAGs含量减少（P<0.01）；P-PC组，弹性纤维（P<0.01）、GAGs（P<0.05）含量均有所增加；P-SIS组，弹性纤维（P<0.001）、GAGs（P<0.05）含
量均增加。结论：P-SIS材料中，弹性纤维和GAGs的再生状况优于其他两组，且P-SIS材料不易引起腹腔内粘连，故P-SIS能够更
好的应用于组织缺损的治疗，P-SIS在组织工程学领域相对而言是更加理想的修复材料。 |
| 英文摘要: |
| Objective:The purpose of this study is to research the degradative regularity about three porcine-derived extracellular
matrix (ECM): small intestine submucosa (P-SIS), pericardium (P-PC) and acellular dermal matrix (P-ADM), by quantitating the change
of elastin, glycosaminoglycans (GAGs) in mouse model at 4 weeks and 8 weeks after surgery. Therefore, our study can provide the theory
for searching an excellent biological mesh, and point the way for clinical application and avoiding postoperative complications of
biological mesh.Methods:60 BALB/c mouse, weighting 18 ~20 g, Abdominal wall defects (1 cm × 1 cm )were created by surgery and
were repaired with the same area of P-SIS, P-ADMand P-PC, randomly divided into three groups (n=20), The animals were sacrificed at
4 and 8 weeks after surgery.Results:The scores of abdominal adhesion in P-SIS group were lower than in the other two groups. In
comparison between 4 weeks and 8 weeks after surgery, GAGs in P-ADMgroups were decreased(P<0.01); elastin(P<0.01）, GAGs(P<0.
05) in P-PC groups were increased; elastin (P<0.001）, GAGs (P<0.05) in P-SIS groups were all increased.Conclusion:The regeneration
of elastin and GAGs in P-SIS groups was better than in the other two groups, and P-SIS was good for anti-adhesion, relatively, so P-SIS
could be applied in the treatment of tissue defect preferably, and it was an ideal material for tissue engineering application. |
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