| 赵慧慧 王道艳 王春波.胶原蛋白肽经由Nrf2信号通路对H2O2诱导的肝细胞
氧化损伤的保护作用（英文）[J].,2014,14(23):4434-4439 |
| 胶原蛋白肽经由Nrf2信号通路对H2O2诱导的肝细胞
氧化损伤的保护作用（英文） |
| Collagen Peptides Protects Human Hepatocytes Against Hydrogen Peroxide(H2O2)-inducedOxidative Stress Damage: Involvement of the Nrf2 Transcription Factor |
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| DOI： |
| 中文关键词: 胶原蛋白肽 过氧化氢 HL7702 抗氧化酶 Nrf2 |
| 英文关键词: Collagen Peptides Hydrogen peroxide HL7702 cells Antioxidant enzymes Nrf2 |
| 基金项目:山东省自然科学基金项目(ZR2011HM046) |
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| 中文摘要: |
| 目的：氧化应激在肝脏疾病中扮演着重要的角色。胶原蛋白肽是天然的抗氧化剂，其在动物实验中已经被证实有抑制氧化
应激的作用。最新研究证实胶原蛋白肽将有可能被应用在肝脏疾病的预防中，但是很少有研究报道其分子作用机制。因此本研究
在胶原蛋白肽是对H2O2诱导的正常人的肝细胞系HL7702 氧化损伤有保护作用的基础上，并探索其分子作用机制。方法：实验设
空白对照组，H2O2模型组，胶原蛋白肽低、中、高剂量组(10, 100, 200 ug/ml)。胶原蛋白肽各组加入相应浓度的药物预处理12 h后，
与模型组一起加入300 uMH2O2的H2O2共同培养12 h，空白对照组正常培养。细胞毒性是由CCK8 和乳酸脱氢酶（LDH）的释放
检测。抗氧化试剂盒检测细胞内活性氧的水平，超氧化物歧化酶（SOD）、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的变化。
Western blot 检测细胞内Nrf2 蛋白的表达水平。结果：胶原蛋白肽对H2O2诱导的正常人的肝细胞系HL7702氧化损伤有保护作
用。胶原蛋白肽能够及时清除细胞内的活性氧，增加Nrf2 的蛋白表达水平，提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活
性，减轻脂质过氧化反应，从而保护正常人的肝细胞系HL7702。结论：总之，胶原蛋白肽通过增加Nrf2 的蛋白表达水平，提高抗氧
化活性，对H2 O2 诱导损伤的肝细胞发挥保护作用。本研究为胶原蛋白肽的分子作用机制提供了新的证据，将有助于预防氧化应激
所致的肝损伤。 |
| 英文摘要: |
| Objective:Oxidative-stress damage has been proved to play an important role in the liver injury. Collagen Peptides are
the natural substance and they are also antioxidant that reduce oxidative stress in animals. Recent years Collagen Peptides have been
proposed for treatment of liver injury, but there are few studies about the molecular mechanism of the protective effect. This study was to
investigate whether Collagen Peptides against hydrogen peroxide ( H2O2)-induced oxidative stress damage in a normal human hepatocyte
cell line, and explore the mechanism of the protective effect of Collagen Peptides.Methods:Five groups including control, H2O2 model,
Collagen Peptides 10 ug/ml, Collagen Peptides 100 ug/ml, Collagen Peptides 200 ug/ml groups were set up in this study. Cells were
cultured with blank control medium or Collagen Peptides of the fixed dosages for 12 h, and then were stimulated with 300 uM H2O2 for
12 h.CCK8 assay and lactate dehydrogenase (LDH) release were analyzed cytotoxicity. Superoxide dismutase (SOD), catalase (CAT),
reactive oxygen species (ROS) levels, and malondialdehyde (MDA) formation were estimated antioxidant activity and lipid peroxidation.
The expression levels of Nrf2 in HL7702 cells were measured by Western blot.Results:The results showed that Collagen Peptides
protected HL7702 cells against H2O2 -induced oxidative stress damage. The mechanism of the protective effect of Collagen Peptides was
correlated with the promotion of SOD and CAT activity, the scavenging of ROS, and the prevention of lipid peroxidation.Conclusion:In conclusion, the present study proved that Collagen Peptides protected HL7702 cells against H2O2-induced oxidative stress damage
through enabling Nrf2 expression and the antioxidative effect. These results will provide new insight to the molecular mechanism of
Collagen Peptides, which will be benefit for preventing oxidative stress-induced liver injury. |
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