| 胡世颉 李兵 邹西峰 胡学安 张磊 王冰 曹宝萍 罗鹏 吕超 费舟.传输靶向COX-2 siRNA联合化疗药物对大鼠胃癌细胞生长[J].,2014,14(24):4634-4636 |
| 传输靶向COX-2 siRNA联合化疗药物对大鼠胃癌细胞生长 |
| Effects of COX2 siRNA Combined with Chemotherapeuticson the Inhibition of Gastric Cancer Cells in Rats |
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| DOI： |
| 中文关键词: 传输siRNA 化疗药物 胃癌 COX-2 蛋白 抑瘤作用 |
| 英文关键词: Transport siRNA Chemotherapy drugs Gastric cancer COX-2 protein Antitumor effect |
| 基金项目:国家自然科学基金青年科学基金项目（81101710） |
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| 中文摘要: |
| 目的：通过动物实验探讨传输靶向COX-2 siRNA联合化疗药物对大鼠胃癌细胞生长的抑制作用。方法：24 只健康SD 大鼠
平分为三组，治疗组用COX-2-siRNA转染的胃癌SGC7901 细胞接种，同时进行环磷酰胺、丝裂霉素C 化疗治疗；阴性对照组，用
阴性对照siRNA 转染的胃癌SGC7901 细胞接种，同时进行环磷酰胺、丝裂霉素C 化疗治疗；对照组(n=8)，用未经转染的胃癌
SGC7901 细胞接种，不进行化疗治疗；三组转染后都接种了裸鼠。结果：治疗组、阴性对照组及对照组胃癌细胞凋亡率分别为
（22.28± 0.12）%、（1.23± 0.17）%和（1.03± 0.14）%，治疗组与阴性对照组和对照组比较差异都有统计学意义(t=18.152，17.555，
P<0.05)。治疗组的抑瘤率为76.7%，阴性对照组和对照组分别为12.8%和6.89%，治疗组的抑瘤率明显高于其他两组(x2=15.
211，13.899，P<0.05)。Western blotting检测结果显示治疗组的COX-2 蛋白表达含量得到了明显抑制。结论：传输靶向COX-2
siRNA和化疗药物的配合应用可有效抑制COX-2 蛋白的表达，从而抑制胃癌细胞的生长，从而起到更好的治疗效果。 |
| 英文摘要: |
| Objective:To investigate the effects of COX2 siRNA combined with chemotherapeutics on the inhibition of gastric
carcinoma cells of rats.Methods:24 healthy SD rats were selected equally divided into three groups, which including the treatment group
(n=8) that were given the SGC7901 cells with COX-2-siRNA transfection and treated by the cyclophosphamide and mitomycin C in
chemotherapy; the negative control group (n=8) that were given the negative control siRNA transfected cells with gastric cancer
SGC7901, and treated by the cyclophosphamide and the mitomycin C in chemotherapy; the control group (n=8) that were given the
untransfected SGC7901 without the chemotherapy; Then the mice in the three groups were vaccinated.Results:The rate of the cells'
apoptosis in the three groups was (22.28± 0.12)%, (1.23± 0.17)% and (1.03 ± 0.14)% , respectively with statistically significant
differences (t=18.152, 17.555, P<0.05). The rate of antitumor in the three groups was 76.7 %, 12.8% and 6.89%, respectively. The
inhibition rate in the treatment group was significantly higher than those of the other two groups (X2=15.211,13.899, P<0.05). Western
blotting analysis showed that COX-2 protein expression levels in the treatment group was significantly inhibited.Conclusion:Transmission targeting COX-2 siRNA combined with the chemotherapy drugs can effectively inhibit the expression of COX-2 protein,
thereby inhibite the growth of gastric cancer cells, which played better therapeutic effect. |
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