| 李继红 何倩 石咏梅 李亮 殷坚 刘超群 陈安 余清平.银杏内酯对大鼠急性脊髓损伤保护作用的实验研究[J].,2014,14(26):5027-5030 |
| 银杏内酯对大鼠急性脊髓损伤保护作用的实验研究 |
| Experimental Study of Protective Effect of Ginkgolide in the Acute SpinalCord Injury in Rats |
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| DOI： |
| 中文关键词: 银杏内酯 脊髓损伤 NF-kB COX-2 |
| 英文关键词: Ginkgolide Spinal cord injury NF-kB COX-2 |
| 基金项目:湖南省科技厅资助项目（2011FJ3141）；湖南中医药大学校青年科研课题项目（99820001-39） |
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| 中文摘要: |
| 目的：探讨银杏内酯对大鼠急性脊髓损伤的保护作用。方法：选取健康成年正常SD 大鼠54 只，分正常组、损伤组和银杏内
酯治疗组；采用改良Allen’s打击法制作脊髓损伤动物模型，分别在伤后6 h、12 h、24 h、72 h处死动物，采用免疫组织化学方法结合图像分析技术观测NF-κB 和COX-2 在脊髓腰段的表达情况。结果：脊髓神经功能评定显示银杏内酯治疗组大鼠神经功能较单纯损伤组有所改善；正常脊髓前角内NF-κB 和COX-2 有一定的基础表达。脊髓损伤后6 h脊髓神经元的胞浆及胞核内NF-κ B和COX-2 均先后表达上升，24 h达高峰，72 h仍维持在较高水平；而给予银杏内酯治疗后，各时间点NF-κB 和COX-2 的表达上调幅度均降低。结论：急性脊髓损伤后，银杏内酯可通过控制NF-资B 和COX-2 的表达上调的幅度而抑制炎症反应，对脊髓受损神经元起一定的保护作用。 |
| 英文摘要: |
| Objective:To investigate the effects of ginkgolide in experimental spinal cord injury (SCI) in rats.Methods:54
healthy adult SD rats were distributed into normal group, injured group and ginkgolide treatment group. The model of was established by
Allen’s method at the level of L3-L5 with a moderate degree of damage. Ginkgolide was administered intraperitoneally immediately after
operation in the ginkgolide treatment group. Rats were survived for 6, 12, 24 and 72 hours after operation. The expression and
distribution of NF- kB and COX-2 in the lumber spinal cord were detected by immunohistochemical method and image analysis
technique.Results:Nervous function of spinal cord in the ginkgolide treatment group was better than that in injured group after SCI in
rats. NF-资B and COX-2 were expressed in normal anterior horn of spinal cord. The expression of NF-kB and COX-2 in neurons of spinal
cord both began to up-regulate at 6 h, peaked at 24 h after SCI in order, still higher than normal level at 72 h . Up-regulated degree of
expression in ginkgolide treatment group was lower than that in injured group after SCI in rats.Conclusion:Ginkgolide can inhibit the
inflammatory reaction to protect injured neurons of spinal cord through the control of up-regulated degree of expression of NF-kB and
COX-2 in the spinal cord after acute SCI in rats. |
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