| 党丹 吴芳 郭俊 李宏增 赵聪 汪杰 李川李柱一.非肥胖型糖尿病小鼠成年神经发生的实验研究[J].,2014,14(29):5605-5608 |
| 非肥胖型糖尿病小鼠成年神经发生的实验研究 |
| Study on Adult Neurogenesis in an Animal Model of Non-obese Diabetes |
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| DOI： |
| 中文关键词: 糖尿病 非肥胖型 神经干/ 祖细胞 增殖 |
| 英文关键词: Diabetes mellitus Non-obese Neural stem/progenitor cell Proliferation |
| 基金项目:国家自然科学基金项目（31200665） |
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| 中文摘要: |
| 目的：探讨异常免疫反应介导的糖尿病（DM）对大脑神经干/ 祖细胞（NSC/NPC）增殖的影响。方法：依据尿糖测定结果将
非肥胖型糖尿病（NOD）小鼠分为发病组和未发病组，观察两组小鼠的体重变化和胰岛炎的严重程度。通过5- 溴-2'- 脱氧尿苷
（BrdU）掺入和免疫荧光染色方法，观察两组小鼠海马齿状回（DG）和侧脑室下区（SVZ）的BrdU 阳性细胞数量的变化，以评估
NSC/NPC增殖情况。结果：发病组小鼠呈现出多饮、多食、多尿等糖尿病典型症状，体重较未发病组明显减低。发病组小鼠胰腺组
织中炎症评分为3 分的胰岛数量明显多于未发病组，且胰岛炎症的平均评分显著高于未发病组（P<0.05）。与未发病组比较，发病
组小鼠大脑海马DG区BrdU阳性细胞显著降低（P<0.01）；SVZ区的BrdU阳性细胞数量亦明显少于未发病组（P<0.001）。结论：
异常免疫反应介导的DM可抑制小鼠大脑NSC/NPC 的增殖。 |
| 英文摘要: |
| Objective:To investigate the changes of the proliferation of neural stem/progenitor cell (NSC/NPC) in diabetes
mellitus (DM) mediated by abnormal immune responses.Methods:Female non-obese diabetic (NOD) mice were divided into non-DMor
DM groups according to the results of glycosuria tests. The changes of body weight and the severity of insulitis were observed and
compared between the two groups. 5-bromo-2'-deoxyuridine (BrdU) incorporation and subsequent BrdU immunofluorescent staining
were used. The NSC/NPC proliferation was assessed by counting the number of BrdU-positive cells in the dentate gyrus (DG) of
hippocampus and in the subventricular zone (SVZ), respectively.Results:Diabetic NOD mice exhibited the classical symptoms of
hyperglycemia including polydipsia, polyuria, and weight loss. The number of islets with an inflammatory score of 3 was larger in DM
group than in non-DMgroup. The mean inflammatory score of insulitis is also higher in DMgroup (P < 0.05). Compared with non-DM
NOD mice, the number of BrdU-positive cells was significantly decreased in the DG of hippocampus in diabetic NOD mice (P<0.01).
Similarly, a reduced number of BrdU-positive cells also occurred in the SVZ of diabetic NOD mice (P<0.001).Conclusion:Abnormal
immune responses-mediated DMcontributes to an impaired NSC/NPC proliferation in female NOD mice. |
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