| 刘彬 张竞之 刘少军 邱怀娜 郭景新 刘慰华.LOX-1 过表达诱发H9C2 心肌细胞肥大[J].,2014,14(29):5644-5647 |
| LOX-1 过表达诱发H9C2 心肌细胞肥大 |
| Lectin-Like Oxidized LDL Receptor-1 Overexpression Induced H9C2Cardiomyocyte Hypertrophy |
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| DOI： |
| 中文关键词: 凝集素样氧化低密度脂蛋白受体-1 心肌细胞肥大 慢病毒载体 |
| 英文关键词: Lectin-like oxidized LDL receptor-1 Cardiomyocyte hypertrophy Lentiviral vector |
| 基金项目:国家自然科学基金项目（81302892）；广东省自然科学基金项目（S2013040016226）；中国博士后基金（2013M530363）；广州医学院博士、留学归国人员基金项目（2012C50） |
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| 中文摘要: |
| 目的：构建携带过表达大鼠凝集素样氧化低密度脂蛋白受体-1（LOX-1）基因的慢病毒载体，研究LOX-1 与心肌细胞肥大的
关系。方法：构建大鼠LOX-1 基因pHIV-LOX-1 过表达质粒，与包装质粒psPAX2、pMD2G共转染293T细胞，检测其侵染效率。
包装慢病毒并侵染H9C2 心肌细胞，72 h后观察其侵染效率。qPCR法检测细胞LOX-1 表达。检测过表达LOX-1 后心肌细胞面积
及其蛋白含量变化。结果：成功构建过表达LOX-1 H9C2 心肌细胞。过表达LOX-1 组(Lv.LOX-1+)心肌细胞面积（16691.890 ±
1022.368 um2）较对照组(Lv.NC)（3459.865 ± 343.175 um2）显著增加（P<0.001）。Lv.LOX-1+ 组心肌细胞蛋白含量(132.457 ±
8.188 pg/cell )较Lv.NC组(45.095 ± 1.655 pg/cell )显著增加（P<0.001）。结论：LOX-1 过表达能诱导H9C2 心肌细胞肥大。 |
| 英文摘要: |
| Objective:To construct the lentiviral overexpression vector of Lectin-like oxidized LDL receptor-1 (LOX-1) and
investigate its correlation with cardiomyocyte hypertrophy in H9C2 Cells.Methods:The lentiviral vector was constructed by inserting
the lentiviral vectors with the LOX-1 gene fragment. The recombination lentiviral particles were produced by the packaging 293T cell.
The culture supernatant was harvested and the titration of them was calculated by the limiting dilution. H9C2 cells were infected with
recombinant lentivirus overexpressing LOX-1 for 72 hours, then transduction efficiency was investigated and LOX-1 mRNA level in cells
was examined by the qPCR. The cell size was measured by immunofluorescence. The cell protein content was examined by BCA kit.Results:Overexpressing LOX-1 H9C2 cardiomyocytes were constructed successfully. The cell size of LOX-1 overexpression group (Lv.
LOX-1+)（16691.890 ± 1022.368 um2）was remarkably increased compared with control gruop (Lv.NC)（3459.865± 343.175 um2）
（P<0.001）. The protein content was enhanced after LOX-1 overexpression (132.457 ± 8.188 pg/cell) compared with control group
(45.095 ± 1.655 pg/cell)(P<0.001).Conclusion:LOX-1 overexpression can induce cardiomyocytes hypertrophy in H9C2 cells. |
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