| 任秋颖 邢舰誉 张宇 王屹博 丁超 李妍 史久慧 刘忠华.BMP7对小鼠诱导多能干细胞骨向分化作用的研究[J].,2014,14(29):5651-5654 |
| BMP7对小鼠诱导多能干细胞骨向分化作用的研究 |
| The Effect of BMP7 on Osteogenetic Differentiation of Mouse InducedPluripotent StemCells |
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| DOI： |
| 中文关键词: 诱导多能干细胞 拟胚体 BMP7 骨向分化 |
| 英文关键词: Induced pluripotent stemcells Embryoid bodies BMP7 Osteogenetic differentiation |
| 基金项目:哈尔滨医科大学心肌缺血省部共建教育部重点实验室开放课题基金（KF201317） |
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| 中文摘要: |
| 目的：探讨骨形态蛋白（bonemorphogenetic protein，BMP）超家族成员之一BMP7在小鼠诱导多能干细胞（Induced pluripotent
stemcells，iPS）骨向分化过程中的作用。方法：本试验分成三组，分别是自发分化组，骨诱导组和添加BMP7 的骨诱导组。每天观
察各组细胞形态学特征及生长状况的差异，在诱导第14 天通过茜素红染色检测基质矿化情况，判断BMP7 在体外骨诱导条件下
对小鼠iPS细胞骨向分化过程所发挥的作用。结果：完成了小鼠iPS 细胞的培养鉴定，并诱导形成理想状态的拟胚体(Embryoid
body,EB)用于分化接种。结果发现，添加BMP7 的骨诱导组细胞的矿化结节阳性率明显增加。结论：BMP7 在诱导小鼠iPS细胞骨
向分化过程中起促进作用，而对非骨向分化的细胞无成骨促进作用。 |
| 英文摘要: |
| Objective:To investigate the effect of BMP7, which is a member of bone morphogenetic protein (BMP), in the process
of mouse induced pluripotent stem cells（iPS）osteogenetic differentiation.Methods:The study focuses on improving mouse iPS cells
differentiating ability into osteoblast in the condition of physiological sugar. Three groups were setted in the study, Spontaneous
differentiation group, bone induction group and BMP7 suplied bone induction group. The morphological characteristics and different
growth status of the cells were observed under the inverted-microscrope every day. The matrix mineralization were detected by alizarin
red staining on the 14th day, so that the function of BMP7 in inducting muose iPS cells into osteoblast in the condition of physiological
sugar were reflacted.Results:The amplification and identification of the mouse iPS were completed, and then the cells were induced into
embryoid bodies (EB), and mineralized nodules were positive and the quantity of the mineralized nodules increased in BMP7 suplied
physiological sugar group.Conclusion:BMP7 can promote osteoblast induction of mouse iPS cells |
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