| 赵佳瑞 白秀峰 杨珂 罗燕 郭海英.Wnt3a在毛囊及黑素细胞谱系中的表达[J].,2014,14(30):5814-5817 |
| Wnt3a在毛囊及黑素细胞谱系中的表达 |
| Expression of Wnt3a in Hair Follicle and Melanocyte Lineage |
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| DOI： |
| 中文关键词: Wnt3a 毛囊 黑素干细胞 黑素细胞 分化 |
| 英文关键词: Wnt3a Hair follicle Melanocyte stemcell Melanocyte Differentiation |
| 基金项目:国家自然科学青年基金项目（81301350）；重庆市面上项目（cstc2013jcyjA10021） |
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| 中文摘要: |
| 目的：探讨毛囊周期中，Wnt3a 在毛囊及黑素细胞中的表达变化。方法：以DCT-LacZ 转基因小鼠为动物模型，通过X-gal
染色技术观察黑素细胞谱系在小鼠皮肤中的分布情况；采用X-gal染色结合免疫组化方法检测Wnt3a 在毛囊及黑素细胞谱系中
的表达情况；采用RT-PCR方法对小鼠皮肤全层Wnt3a 和TYR 的mRNA 表达进行半定量分析。结果：在生长期毛囊中，Wnt3a
蛋白在表皮、毛囊外根鞘Bulge 区、内根鞘以及毛球部均有表达，在黑素干细胞与黑素细胞也观察到Wnt3a；在退化期，Wnt3a 的
表达逐渐减弱，仅在外根鞘有较弱的表达，但黑素干细胞中没有观察到Wnt3a；在静止期，几乎检测不到Wnt3a 的表达；TYR
mRNA与Wnt3a mRNA 在毛囊周期中的表达模式一致，在生长期最强，退化期减弱，静止期最弱。结论：Wnt3a 可能对黑素细胞
谱系分化起到促进作用。 |
| 英文摘要: |
| Objective:To examine the presence and location of Wnt3a in hair follicle and melanocyte lineage during hair cycle.Methods:By using Dct-LacZ transgenic mice, the protein expression pattern of Wnt3a was observed by X-gal staining and
immunohistochemistry in the slides of skin in hair cycle. The mRNA expression quantity of Wnt3a and tyrosinase were observed by
RT-PCR in the whole skin in hair cycle.Results:Wnt3a was expressed in the epidermis, bulge, IRS precursors and hair bulb in anagen
hair follicle, decreased in catagen, and was basically not expressed in telogen. Melanocyte stem cells and melanocytes also expressed
Wnt3a in anagen hair. The mRNA expression pattern of Wnt3a and tyrosinase were consistent, which were most strongly expressed in
anagen, decreased in catagen, and lowest in telogen.Conclusion:Wnt3a may promote melanocyte lineage differentiation. |
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