| 梁晶 刘惠彬 李哲 刘冬敏 孙向菊 吴禹蒙 张鑫 吴玉波.HERG钾通道在细胞容量调节中的作用[J].,2014,14(32):6253-6256 |
| HERG钾通道在细胞容量调节中的作用 |
| The Contribution and Mechanismof the HERG PotassiumChannels on theRegulation of Cell Volume |
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| DOI： |
| 中文关键词: HERG钾通道 膜片钳 细胞容量 调节性细胞容量下降 |
| 英文关键词: HERG potassiumchannels Patch-clamp techniques Cell volume |
| 基金项目:中国博士后科学基金项目（2012M520775） |
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| 中文摘要: |
| 目的：研究HERG 钾通道在细胞容量调节中的作用，并探讨其作用机制。方法：全部试验应用稳定转染HERG-HEK293 细胞
和HEK293细胞。应用全细胞膜片钳技术记录HERG钾电流。结果：①当HERG-HEK293 细胞处于低渗状态，指令电压为0 mV
时，Istep 增加60%（n=12，P<0.05）；如指令电压为+30 mV，Itail 增加72.1%（n=11，P<0.01)，增大的HERG 电流可被特异性HERG
钾电流阻断剂Cisapride（100nM）抑制，Istep 被抑制97.2%(n=6，P<0.01)，Itail 被抑制174.1%(n=6，P<0.01)。②在低渗状态，指令电
压为0 mV 时，容量调节性氯通道阻断剂尼氟灭酸（NFA，10 nmol/L）使Istep 抑制46.2%（n=12，P<0.01)，Itail 抑制48.5%（n=11，
P<0.01）；给以容量调节性氯通道阻断剂DIDS 100 滋mol/L时，Istep 抑制45.9%（n=12, P<0.01)，Itail抑制51.1%（n=11，P<0.01）。结
论：HERG通道部分参与调节性细胞容量下降过程。其参与的细胞容量调节与容量调节性氯通道的激活相伴随。 |
| 英文摘要: |
| Objective:To investigate the effect of HERG (Human ether-a-go-go related gene) potassium channels on the
regulatory volume decrease and to reveal the possible mechanism.Methods:The stable HERG-transfected HEK293 cell line and HERG
non-transfected HEK293 cell line were used for all the experiments, HERG potassium current was recorded in whole-cell patch-clamp
techniques.Results:① When the HERG-HEK293 cells are exposed to the hypotonic solution and the test potential is 0mV, the Istep
increased 60%(n=12, P<0.05); the Itail increased 72.1% (n=11, P<0.01); the increase currents can be inhibited by the specific blocker
Cisapride (100nmol/L), Istep can be inhibited by 97.2% (n=6, P<0.01) and the Itail can be inhibited by 174.1% (n=6, P<0.01). ②
Exposure to the hypotonic solution and in the presence of the volume regulated chloride channel blockers Niflumic Acid (10 nmol/L), the
Istep can be inhibited by 46.2% (n=12, P<0.01) and the Itail can be inhibited by 48.5% (n=11, P<0.01); In the presence of DIDS (100
滋mol/L), the Istep can be inhibited by 45.9% (n=12, P<0.01) and Itail can be inhibited by 51.1%(n=11, P<0.01).Conclusion:HERG
potassium channels are partly involved in the process of the regulatory volume decrease, and the involvement followed the activation of
the volume regulated chloride channel. |
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