| 周建宇 毛立民 王巍 吕少华 赵晶.MS-275 联合平阳霉素对Tca-8113 细胞生长和凋亡的影响[J].,2014,14(33):6407-6410 |
| MS-275 联合平阳霉素对Tca-8113 细胞生长和凋亡的影响 |
| Effect of Combined Application of Histone Deacetylase Inhibitors (HDACIs)MS-275 and Pingyangmycin (PYM) on the Growth and Apoptosis of OralSquamous Carcinoma Cell |
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| DOI： |
| 中文关键词: MS-275 平阳霉素 口腔鳞状细胞癌 细胞凋亡 细胞增殖 |
| 英文关键词: MS-275 Pingyangmycin Oral squamous carcinoma Cell apoptosis Cell proliferation |
| 基金项目: |
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| 中文摘要: |
| 目的：观察组蛋白去乙酰化酶抑制剂(HDACIs)MS-275 联合抗生素类化疗药物平阳霉素(PYM)对口腔鳞状癌细胞Tca-8113
的生长及凋亡的影响。方法：以体外培养的口腔鳞状细胞癌Tca-8113 细胞为研究对象，应用MTT 法检测不同浓度(0、1、2、4、8
μmol/L)MS-275、(0、0.05、0.1、0.2、0.4 μmol/L) 平阳霉素(PYM) 单独和联合用药对Tca-8113 细胞增殖活性的影响；
Annexin-V-FITC/PI 双染流式细胞术定量检测细胞的凋亡情况。结果：不同浓度(1、2、4、8 μmol/L)MS-275 和(0.05、0.1、0.2、0.4
μmol/L)PYM 均可显著抑制Tca-8113 细胞的增殖，并促进其凋亡，且呈浓度依赖性(P<0.05)。4 μmol/L MS-275 和0.2 μmol/L PYM
联合应用时，其抑制Tca-8113 细胞增殖和促进其凋亡的作用均显著高于单独应用MS-275 或PYM(P<0.05)。结论：MS-275能有
效增强口腔鳞状细胞癌Tca-8113 细胞对平阳霉素(PYM)的敏感性，体外实验中呈现较好的抗肿瘤效应。 |
| 英文摘要: |
| Objective:To investigate the influence of combined application of histone deacetylase inhibitors MS-275 and
antibiotics, chemotherapy drugs Pingyangmycin on the growth and apoptosis of oral squamous carcinoma cell Tca-8113.Methods:Oral
squamous cell carcinoma Tca-8113 cells cultured in vitro were used for the study, MTT was performed to examine the proliferative
activity of Tca-8113 cells after separated or combined application of (0, 1, 2, 4, 8μmol/L ) MS-275and (0, 0.05, 0.1, 0.2, 0.4 μmol/L)
PYM; Annexin V-FITC/PI double dye, Flow cytometry was used to detect the effects of MS-275 plus PYMon the apoptosis of Tca-8113
cells.Results:Different concentrations(1, 2, 4, 8 μmol/L) of MS-275 or (0.05, 0.1, 0.2, 0.4 μmol/L) PYMcould both inhibit the growth
and induce the apoptosis of Tca-8113 cells at a concentration dependent manner (P<0.05). Compared with MS-275 or PYM alone, 4
μmol/L MS-275 plus 0.2 μmol/L PYM could more significantly inhibit the proliferation of Tca-8113 cells and promote the apoptosis
(P<0.05).Conclusion:MS-275 could effectively enhance the sensitivity of Tca-8113 cells to PYM, which showed good anti-tumour
effect in vitro. |
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