| 辛雨 葛银林 郑征 刘永超.n-3 多不饱和脂肪酸调节饮食诱导肥胖大鼠 miRNA 表达变化[J].,2014,14(35):6830-6834 |
| n-3 多不饱和脂肪酸调节饮食诱导肥胖大鼠 miRNA 表达变化 |
| Identification of n-3 PUFA-modulated miRNA Expression Pattern inDiet Induced Obesity Rats |
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| DOI： |
| 中文关键词: n-3 多不饱和脂肪酸 肥胖 miRNA 大鼠 |
| 英文关键词: N-3 polyunsaturated fatty acids Diet Induced Obesity MiRNA Rats |
| 基金项目:山东省自然科学基金（ ZR201 0CM010） |
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| 中文摘要: |
| 目 的： 研究 n-3 多不饱和脂肪酸（ polyunsaturated fatty acids, PUFA）饮食对饮食诱导肥胖大鼠的 miRNA 表达影响。 方法： 将
10 只饮食诱导肥胖(diet induced obese, DIO)大鼠随机分成两组： n-3PUFA 添加组和安慰剂添加组（对照组）； 每周记录两组老鼠的
体重、体长和进食量。 对外周 血 miRNA 的表达并进行分析和预测。 结果： 两组老鼠 Lee 指数有统计学差异（ P<0.05）；与 对照组相
比，在 n-3 组的外周 血单核细胞中， 29 个 miRNA 上调， 31 个下调； 其中 rno-miR-200 和 rno-miR-211 的 表达量上调， rno-miR-29b
和 rno-miR-92b 的表达量下调， 其靶基因 预测结果与 神经营养因 子， 脂肪细胞因子， 趋化因子和胰岛素信号通路有关。 结论：
n-3PUFA 能够调节 DIO 大鼠的 miRNA 水平， 其中有些与 脂肪代谢相关。 |
| 英文摘要: |
| Objective:To determine the effect of n-3 PUFA in the miRNA level in diet induced obese (DIO) rats.Methods:A
total of 1 0 DIO rats were randomly divided into two groups: n-3 PUFA-containing group and PBS-containing group (control group).
Body weight, body length and food intake were recorded once a week. Small RNA was extracted from PBMCs of the two groups for
miRNA array assay. Four of the identified differentially expressed miRNAs were selected and verified using real-time PCR. Then we
predicted the target genes of the miRNAs with miRWalk. The predicted target genes were analyzed by using DAVID database and Kyoto
Encyclopedia of Genes and Genomes (KEGG) biological pathway using the molecular annotation.Results:The lee's index of two groups
in the 10th week had statistical difference. 29 up-regulated miRNAs and 31 down-regulated miRNAs were identified in the PBMCs. The
expression of rno-miR-200 and rno-miR-21 1 in the peripheral blood mononuclear cells (PBMCs) were up-regulated in rats of n-3 group,
while rno-miR-29b and rno-miR-92b down-regulated. Target genes of the four miRNAs were significantly involved in neurotrophin,
adipocytokine, chemokine, and insulin signaling pathways.Conclusion:n-3 PUFA can regulate the miRNA level on adipose tissue
metabolism in DIO rats. |
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