| 肖宏 胡炜 张涵 陈赤丹 王来藏 任付宾 王宏伟.ABCG2 与胶质瘤血管形成及预后的相关研究[J].,2014,14(35):6916-6922 |
| ABCG2 与胶质瘤血管形成及预后的相关研究 |
| Correlative Research of ABCG2 with Angiogenesis and Prognosisin Patients of Glioma |
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| DOI： |
| 中文关键词: 脑胶质瘤 组织芯片 免疫组化 预后 |
| 英文关键词: Brain Gliomas Tissue Microarray Immunohistochemical Prognosis |
| 基金项目:黑龙江省教育厅科学技术研究项目（ 1251 1257） |
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| 中文摘要: |
| 目 的： 研究 ABCG2 在胶质瘤血管形成过程中与 VEGF、 VEGFR 和 CD34 的关系， 探讨其在血管形成中的作用 及对胶质瘤
患者生存预后的影响。 方法： 采用 脑胶质瘤的 组织芯片 技术， 分析 ABCG2、VEGF 和 VEGFR(flt-1)在胶质瘤中的表达率， 根据
CD34 阳性的血管计数判定微血管密度（ MVD）； 另 用 免疫荧光共聚焦检测 ABCG2 与 CD34、VEGF 的共表达；用 COX 回归模型
分析 ABCG2 对胶质瘤患者预后影响。 结果： ABCG2、 VEGF 、 VEGFR(flt-1) 和 CD34 阳性表达率随着胶质瘤恶性程度的增加而增
高。 ABCG2Ⅰ 、Ⅱ 级之间无统计学差异， 其余各级别 之间存在统计学差异（ P<0.05）； ABCG2 与 病理级别呈正相关； ABCG2 表达水
平与 MVD 显著相关， 酌＝ 0.540， P<0.001。 ABCG2、VEGF 和 VEGFR (flt-1) 均 为 阳性表达的 肿瘤 标本 MVD 平均 值显著高于
ABCG2 阴 性表达者， P<0.001。 ABCG2 与 CD34、 VEGF 共表达于血管壁。 COX 回归模型证明 ABCG2 是胶质瘤患者预后的危险因
素。 结论： ABCG2 阳性表达细胞具有向肿瘤血管细胞分化的潜能，对肿瘤血管研究重要意义， 且 ABCG2 表达可作为 考察胶质瘤
患者预后的重要指标。 |
| 英文摘要: |
| Objective:To study the relation of ABCG2 in glioma angiogenesis with VEGF, VEGFR and CD34, and investigate its
role in angiogenesis, and to analyze its effect on prognosis of glioma patients.Methods:A tissue microarray technology of glioma was
used to analyze the rate of ABCG2, VEGF and VEGFR (flt-1) expression in glioma. According to the numbers of CD34 positive labeled
microvasculars, tumor microvasculars density (MVD) was calculated. The immunofluorescence confocal was used to detect the
coexpression of ABCG2 and CD34, VEGF in the vessel wall. COX regression model was used to analyze the relation between ABCG2
and prognosis of patients with glioma.Results:The positive expression rate of ABCG2, VEGF, VEGFR and CD34 increased with the
pathological grades. The expression of ABCG2 was statistically different (P<0.05) among each pathological grades except the grade Ⅰ -
Ⅱ . The expression of ABCG2 and pathological grades was positively correlated; moreover, the expression level of ABCG2 was
intimately related with MVD in tumors, 酌＝ 0.540, P<0.001. The MVD in gliomas of grade Ⅲ -Ⅳ was higher than that in gradeⅠ -Ⅱ .
The genes of VEGF, VEGFR (flt-1 ) and CD34 were also analyzed, showing the average value of MVD in tumors expressing VEGF+,
VEGFR+ (flt-1 ) and ABCG2+ was significantly higher than that in the ABCG2- tumors (P<0.001). ABCG2 was co-expressed with CD34
and VEGF in the vessel wall. COX regression model showed that ABCG2 could be as a prognostic factor for the patients with glioma.Conclusion:ABCG2 positive cells have potential to differentiate into the tumor vascular cells, which would be significant for the study
of tumor blood vessels. Furthermore, ABCG2 expression may be an important prognostic indicator in patients with glioma. |
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