| 孙丽萍1 周沫2 徐文静2 金鸿波2 韩薇2.HL-1 细胞培养及房颤细胞模型的建立[J].,2014,14(36):7011-7014 |
| HL-1 细胞培养及房颤细胞模型的建立 |
| Culture of HL-1 Cardiomyocytes and Establishment of Rapid Paced CellsModel |
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| DOI： |
| 中文关键词: 心房颤动 细胞培养 HL-1 细胞 心脏起搏 |
| 英文关键词: Atrial fibrillation Cell culture HL-1 cardiomyocytes Cardiac pacing |
| 基金项目:国家自然科学基金面上项目（81270251） |
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| 中文摘要: |
| 目的：利用HL-1 细胞建立快速起搏模型，对心房颤动(atrial fibrillation, AF）早期的重构现象进行初步研究。方法：培养HL-1
细胞，建立快速电场刺激起搏细胞模型，利用全细胞膜片钳技术记录刺激前后HL-1 细胞的动作电位周期，透射电镜观察细胞超
微结构的变化。结果：将细胞接种于培养皿中，72 h后细胞呈融合状态，全细胞膜片钳记录培养HL-1 细胞及经电场刺激(600 次
/min, 1 V/cm) 24 h 后的心房肌细胞的动作电位周期，动作电位周期分别为106 ms，45 ms，刺激前后差异有统计学意义(P<0.05)。
透射电镜观察到刺激后HL-1 细胞超微结构发生去分化改变。结论：经快速起搏24 h 后，HL-1 细胞发生了电及结构重构；利用
HL-1 细胞建立快速起搏的房颤模型，可以对房颤早期的重构机制进行研究。 |
| 英文摘要: |
| Objective:To explore early electrical and structural remodeling in atrial fibrillation utilizing a rapid paced cultured
HL-1 cardiomyocytes model.Methods:HL-1 cardiomyocytes were cultured and established as a rapid paced cultured HL-1
cardiomyocytes model. HL-1 cells were cultured in the presence of rapid field stimulation (600 beats per min,1 V/cm)for 24 h. Action
potential duration was recorded fromstimulated cells and control cells using whole-cell voltage-clamp techniques. Ultrastructure of HL-1
cells was observed under electron microscope.Results:After 72h of culture, the cells were in the confluent phase. The action potential
duration was shortened through the rapid pacing compared with those before pacing (P<0.05). Results of electron microscope showed
that HL-1cells generated dedifferentiation through pacing.Conclusion:Rapid stimulation of HL-1 cells in culture produces electrical
remodeling, recapitulating principal phenotypic features of atrial tachycardia remodeling in vivo. It is available that a rapid paced cell
model is established with cultured HL-1 cells to study the mechanismof early electrical and structural remodeling for atrial fibrillation. |
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