| 谢洁1，2 王珊珊2 纪筠1 王涛1 杨晓明1△.右美托咪定对红细胞变形性影响的体外研究[J].,2014,14(36):7049-7051 |
| 右美托咪定对红细胞变形性影响的体外研究 |
| The Effects of Dexmedetomidine on the Deformability of Erythrocytein Vitro |
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| DOI： |
| 中文关键词: 右美托咪定 红细胞变形性 内皮型一氧化氮合酶 一氧化氮 |
| 英文关键词: Dexmedetomidine Erythrocyte deformability Endothelial Nitric Oxide Synthase Nitric oxide |
| 基金项目:空军总医院科研项目（kz2012018） |
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| 中文摘要: |
| 目的：研究右美托咪定（DEX）对离体红细胞变形性的影响，探讨其作用机制。方法：采健康志愿者人血5 mL 制成2 %红细
胞悬液，分成空白对照组（C 组），低浓度DEX 组（DL 组）、中浓度DEX组（DM 组）、高浓度DEX 组（DH 组），单纯育亨宾（Y 组）
以及育亨宾+ DEX组（YD 组），每组9 例。将各组样本放入37 ℃恒温震荡培养箱中60 min 后取出，测定红细胞变形性指数（EI）、
红细胞内一氧化氮（NO）含量以及内皮型一氧化氮合酶（eNOS）含量。结果：与C 组比较，DL组、DM 组、DH 组及YD 组中EI、红
细胞内NO、eNOS 的含量增高（P<0.05）；Y组含量差异无统计学意义（P>0.05）。与YD 组比较，DM组EI、红细胞内NO及eNOS
含量增高（P<0.05）。结论：DEX 可以提高离体红细胞的变形能力，其可能机制是通过激活红细胞膜上肾上腺素受体，激活红细胞
内eNOS使细胞内NO增多有关。 |
| 英文摘要: |
| Objective:To evaluate the impact of clinically relevant concentrations of dexmedetomidine on the deformability of
erythrocyte in vitro.Methods:5 mL intravenous blood samples taken from healthy adults were made into erythrocyte suspensions with 2
% HCT in PBS buffer. Erythrocyte suspensions were divided into 6 groups: control group (group C); low concentration of
dexmedetomidine group (group DL); middle concentration of dexmedetomidine (group DM); high concentration of dexmedetomidine
(group DH); yohimbine alone (group Y) and yohimbine mixed with dexmedetomidine (group YD), with 9 cases in each group. 6 groups
were placed into thermostatic incubator concussion 50 rpm, 37 ℃. After incubation for 60 min, the concentration of NO and eNOS of red
blood cell and deformability of erythrocyte were measured respectively.Results:The EI and the activity of eNOS and content of NO of
erythrocyte were significantly higher in group DL, DM, DH and YD than in groupC (P<0.05). There was no significant difference in the
indexes metioned above between group Y and group C (P>0.05). Compared with group YD, the EI and the activity of eNOS and
concentration of NO of erythrocyte increased in group DM(P<0.05).Conclusion:Dexmedetomidine treatment can improve deformability
of erythrocyte. The improvement effect of dexmedetomidine on erythrocyte deformability is partially associated with adrenergic receptors
through excitement of eNOS to enhance the concentration of NO of red blood cell. |
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