| 祝加学 陆雄伟 沈尊理 秦金保 王 洋 金 晨.脐带间充质干细胞诱导分化为类许旺细胞的实验研究[J].,2015,15(5):834-838 |
| 脐带间充质干细胞诱导分化为类许旺细胞的实验研究 |
| Differentiation of Human Umbilical Cord Mesenchymal Along a SchwannCells Lineage |
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| DOI： |
| 中文关键词: 脐带间充质干细胞 类许旺细胞 诱导 胶质细胞生长因 子 |
| 英文关键词: Umbilical cord mesenchymal stem cells Schwann-like cells Inducen Glial growth factors |
| 基金项目:国家自 然科学基金项目 (30872630) |
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| 中文摘要: |
| 目的： 探讨通过化学诱导的方法， 诱导脐带间 充质干细胞分化为 类许旺细胞， 为组织工程神经寻找一种新的种子细胞来源。
方法： 通过酶消化法， 分离 获得原代脐带间充质干细胞。 体外培养至第 3 代（ P3），以 1× 103/cm2 接种于培养瓶中，待细胞长至亚融
合状态，吸弃培养液， 加入含 茁- 巯基乙醇的培养液预诱导 24 h。 然后， 加入含有全反式维甲酸的培养液进一步诱导 72 h。 最后， 加
入含有胶质细胞生长因 子的培养液， 作用 两周 。对诱导后的脐带间 充质干细胞用 免疫荧光及 RT-PCR 法，进行形态观察和表型鉴
定。 结果： 经过上述诱导过程，脐带间充质干细胞的形态， 由开始时的扁平、片 状、多 极， 类似成纤维细胞状，逐渐变为长梭形，双极
或三极，类似许旺细胞。 同时，表达许旺细胞特异性标志 S-100、 P75；而且 S100， P75 的 mRNA 水平也明显上调， 并且可以观测到
GFAP 的 mRNA 条带。 结论： 化学诱导法， 可以将脐带间充质干细胞诱导分化为 类许旺细胞， 有望为组织工程神经提供一种新的
许旺细胞来源。 |
| 英文摘要: |
| Objective:To explore the potential differentiation of Schwann cells from human umbilical cord mesenchymal stem
cells under the induction of chemical agents, which may provide a new source of seed cells for neural tissue engineering.Methods:The
primary human umbilical cord mesenchymal stem cells were harvested by digestion and isolation. After the 3rd passage, the cells were
plated at 1× 103/cm2 in the flask and treated with 茁-mercaptoethanol for 24h when the cells were subconfluent. Afterwards, the cells were
cultivated with all-trans-retinoic acid for 72h and then incubated with glial growth factors for two weeks. Then the morphology and phenotype of induced umbilical cord mesenchymal stem cells were identified through immunocytochemical staining and RT-PCR.Results:The primary umbilical cord mesenchymal stem cells were flat, platy, multipolar like Schwann cells, but shifted to spindle shape, bipolar
or tripolar like fibroblast cells. Moreover, the specific markers for Schwann cells, S100 and P75, were positively expressed in the induced
cells. The mRNA levels of S100 and P75 also significantly increased along with the expression GFAP.Conclusion:Schwann-like cells
can be induced from umbilical cord mesenchymal stem cells by induction with chemical agents, which has the potential to be a novel cell
source for neural tissue engineering. |
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