| 王 猛 李 楠 李晓祥 徐 奎 李 钊 董 川 文艳华 马 琼 刘 云燕 裘秀春
范清宇 马保安.不同场强下 IRE 的肿瘤消融作用及对抗肿瘤免疫的影响[J].,2015,15(5):849-853 |
| 不同场强下 IRE 的肿瘤消融作用及对抗肿瘤免疫的影响 |
| Tumor Ablation Effect of IRE at Different Parameters and itsTriggering-effect on the Anti-tumor Immunologic Response |
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| DOI： |
| 中文关键词: 不可逆电穿孔 消融 肿瘤 免疫反应 |
| 英文关键词: Irreversible electroporation Ablation Tumor Immunologic response |
| 基金项目:国家自然科学基金项目（ 81 402574）； 国家自然科学基金项目（ 81 272489） |
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| 中文摘要: |
| 目的： 探讨不同 电场强度的不可逆电穿孔（ IRE）对于肿瘤的消融效果以及其所引 起的肿瘤免疫反应。 方法： 将 B16 黑色素瘤
细胞接种于 C57 小鼠，制作黑色素瘤小鼠模型，应用 不可逆电穿孔仪对肿瘤进行消融， 通过测量肿瘤生长大小研究不同 场强下的
消融效果。 通过 CD4、 CD8a 免疫组化染色初步了 解不同 场强激发的免疫反应的强弱， 从而选择最佳消融参数。 结果： 随着电场强
度增加， 肿瘤消融效果逐渐趋于理想，然而平板电极夹之间过高电场引 发的热效应则不利 于后续抗肿瘤免疫反应的激活。 选择合
适的电场， 通过增加电击组数可以达到良好的治疗效果。 结论： IRE 对肿瘤的消融优势在于它能够很好的激活机体的抗肿瘤免疫
反应，因 此，选择合适的电场增加电击组数可以起到很好的抗肿瘤作用， 具有很好的应用 前景。 |
| 英文摘要: |
| Objective:To investigate the tumor ablation effect of Irreversible Electroporation (IRE) at different parameters and its
triggering-effect on the anti-tumor immunologic response.Methods:A melanoma mouse model was established. Then IRE was applied
directly to ablate the tumors at different parameters. After tumor-harvest at different time spots, the tumor sizes of different groups were
determined, and the immunohistochemical staining of CD4 and CD8a were used to detect the anti-tumor immune responses. And finally,
the best parameter which could both kill the tumor and trigger the immunologic response effectively was selected.Results:: The tumor-ablation effect was better with the electrical field increasing. However, higher electrical field resulted in thermal effect, which had strong resistant effect on the anti-tumor immunologic response. Then we selected the lower electrical field and achieved a better effect of tumor
ablation through increasing the number of ablation groups.Conclusion:Compared with traditional thermal ablation techniques, IRE has
better active effect on the immune system. Thus, a proper electrical field and ablation groups can be very effective in tumor-killing. We
believe that IRE will have a great clinical application. |
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