| 杨丽娜 赵静 吴娟 李郁 杨红.Twist1调控FoxM1 在上皮性卵巢癌中的表达[J].,2015,15(8):1452-1456 |
| Twist1调控FoxM1 在上皮性卵巢癌中的表达 |
| Twist1 Regulates the Expression of FoxM1 in Epithelial Ovarian Cancer |
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| DOI： |
| 中文关键词: Twist1 FoxM1 上皮性卵巢癌 表达调控 |
| 英文关键词: Twist1 FoxM1 Human epithelial ovarian cancer Expression and regulation |
| 基金项目:国家自然科学基金项目（81272202） |
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| 中文摘要: |
| 目的：探讨Twist1 调控转录因子FoxM1 在上皮性卵巢癌中的表达。方法：采用免疫组织化学SP法检测FoxM1、Twist1 在上
皮性卵巢癌组织中表达情况,斯皮尔曼秩相关分析其在上皮性卵巢癌组织中的表达相关性；双荧光素酶报告系统检测Twist1 对
FoxM1 的调控作用；Real-time quantitative RT-PCR 和蛋白免疫印迹技术验证Twist1 对FoxM1 的调控作用。结果：Twist1、FoxM1
在上皮性卵巢癌组织中的阳性表达率分别是71.4 %（40/56）、78.6 %（44/56），明显高于正常卵巢组织，差异有统计学意义(P<0.05)；
且其在上皮性卵巢癌中的表达显著相关（r=0.896，P<0.01）；Twist1 可以结合并激活FoxM1 启动子（P<0.05）；上调Twist1 表达可
以激活FoxM1 在卵巢癌细胞中的表达（P<0.05），而干扰Twist1 后可以下调FoxM1 的表达（P<0.05）。结论：Twist1 参与调控增殖
相关转录因子FoxM1 在卵巢癌中的表达，可能是一个潜在的治疗靶点。 |
| 英文摘要: |
| Objective:This study aims to explore that Twist1 regulates the expression of transcription factor FoxM1 in human
epithelial ovarian cancer.Methods:Immunohistochemical staining (SP) was used to evaluate the expression levels of FoxM1 and Twist1
in human ovarian cancer, Spearman's rho was calculated to analyze the correlation between FoxM1 expression and Twist1 expression.
Dual-luciferase reporter assay system was employed to test the effect of Twist1 on the expression of FoxM1. Real-time quantitative
RT-PCR and Western-blot were exerted to validate regulatory effect of Twist1 on FoxM1.Results:The positive expression rates of
Twist1 and FoxM1 in epithelial ovarian cancer were 71.4%（40/56）and 78.6%（44/56）, respectively, which were remarkably higher than
those in normal ovarian tissues. The difference was statistically significant（P<0.01）. FoxM1 had a significant positive correlation with
the expression of Twist1 in these epithelial ovarian cancer samples（r=0.896, P<0.01）.Twist1 was involved in activation of the FoxM1
promoter（P<0.05）. Up regulation of Twist1 can activate expression of FoxM1 in epithelial ovarian cancer（P<0.05）, and the expression
of FoxM1 was decreased significantly when the expression of Twist1 was downregulated（P<0.05）.Conclusion:Twist1 may be involved
in regulation of expression of proliferation-associated transcription factor FoxM1 in human ovarian carcinoma and may serve as a
promising therapeutic target for ovarian cancer. |
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