| 苏敬敬 陶晓晓 舒良 潘辉 翟宇△.Homer1b/c 在缺氧复氧损伤神经元中的保护作用研究[J].,2015,15(19):3605-3608 |
| Homer1b/c 在缺氧复氧损伤神经元中的保护作用研究 |
| Homer1b/c Neuroprotection under Hypoxia/reoxygenation-inducedNeuronal Injury |
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| DOI： |
| 中文关键词: Homer1b/c 缺氧复氧 神经保护 过表达 siRNA干扰 |
| 英文关键词: Homer1b/c Hypoxia/reoxygenation Neuroprotection Over-expression Small interfering RNA |
| 基金项目:国家自然科学基金青年项目（81200941） |
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| 中文摘要: |
| 目的：探讨Homer1b/c 在缺氧复氧损伤神经元中的作用。方法：构建pcDNA3.1-Homer1b/c 质粒和Homer1b/c siRNA，过表达
及siRNA干扰Neuro-2a 细胞中Homer1b/c 的水平，western blot 分析观察上调及下调Homer1b/c 后Homer1b/c 的蛋白表达变化，
再予细胞缺氧复氧损伤处理，观察细胞生存率、乳酸脱氢酶（Lactic dehydrogenase, LDH）释放量及caspase-3 活性的变化。结果：过
表达及siRNA干扰Homer1b/c 后，Homer1b/c 的蛋白水平分别较正常组明显增高及降低(P 值分别<0.01)，提示过表达及干扰
Homer1b/c 的效果均明显，分别能明显提高及降低Homer1b/c 的水平。在缺氧复氧损伤条件下，Homer1b/c 过表达组较空质粒转
染组，其细胞生存率明显增加，LDH 释放量、caspase-3 活性明显降低(P 值分别<0.05)；相反，下调Homer1b/c 的水平后，与control
siRNA转染组相比，细胞生存率明显降低，而LDH 释放量和caspase-3活性明显增加(P 值分别<0.05)。结论：Homer1b/c 在缺氧复
氧损伤神经元中具有神经保护作用。 |
| 英文摘要: |
| Objective:To explore the role of Homer1b/c in hypoxia/reoxygenation-induced neuronal injury.Methods:The
plasmids with pcDNA3.1-Homer1b/c and Homer1b/c small interfering RNA (siRNA) were constructed for over-expression and
down-expression of Homer1b/c in Neuro-2a cells. Western blot analysis was determined to detect Homer1b/c protein levels after
over-expression and down-expression of Homer1b/c. Cells were treated with hypoxia and reoxygenation (hypoxia/reoxygenation). The
changes in cell viability, lactic dehydrogenase (LDH) release and caspase-3 activity were observed.Results:Compared with normal
control group, Homer1b/c protein levels were markedly promoted and reduced in pcDNA3.1-Homer1b/c- and Homer1b/c
siRNA-transfected cells, respectively, suggesting that Homer1b/c over-expression and down-expression were able to up-regulate and
down-regulate Homer1b/c expression (P value<0.01). Under hypoxia/reoxygenation condition, compared with pcDNA3.1-transfetcted
cells, there was a significant increase in cell viability, decreases in LDH release and caspase-3 activity in Homer1b/c over-expression
cells (P value<0.05, respectively). In contrast, compared with control siRNA-transfetcted cells, Homer1b/c down-expression group
produced opposite results, characterized by a significant decrease in cell viability, increases in LDH release and caspase-3 activity (P
value<0.05, respectively).Conclusion:Homer1b/c plays a neuroprotective role in hypoxia/reoxygenation-induced neuronal injury. |
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