| 屈小玲 王文清 梁向艳 铁茹 刘芳娥 李榕 张海锋.罗布麻通过抑制氧化应激反应减轻心肌缺血再灌注损伤[J].,2015,15(19):3609-3612 |
| 罗布麻通过抑制氧化应激反应减轻心肌缺血再灌注损伤 |
| Apocynum Venetum Attenuates MyocardialIschemia/reperfusion Injury via Inhibiting Oxidative Stress |
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| DOI： |
| 中文关键词: 布麻 氧化性应激 心肌缺血再灌注损伤 |
| 英文关键词: Apocynumvenetum Oxidative stress Myocardial ischemia/reperfusion injury |
| 基金项目:国家自然科学基金项目（81270330，81470413，81300190）；陕西省科学技术研究发展计划项目（2013KJXX-89） |
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| 中文摘要: |
| 目的：观察罗布麻叶提取物（apocynumvenetumleaf extract AVLE）预处理对心肌缺血再灌注（MI/R）损伤的影响。方法：采用
SD大鼠MI/R 模型，随机分为sham（假手术）组、MI/R组、AVLE 预处理+MI/R 组，检测血流动力学，采用氯化三苯基四氮唑和伊
文思蓝双染法检测心梗面积、以血浆肌酸激酶（CK）和乳酸脱氢酶（LDH）活性检测心肌损伤情况、以超氧化物、丙二醛（MAD）和
超氧化物歧化酶（SOD）含量检测心肌氧化应激以及Western blot 方法检测gp91phox的表达。结果：与MI/R 组相比，AVLE 预处理
组左室压上升、下降最大速率（± LVdp/dt max）升高（P＜0.05），心肌梗死面积减少，两组分别为41.5± 4.5%和32.0± 3.5%（P＜
0.05），血浆CK和LDH活性分别降低到1653± 62 U/L 和2461± 152 U/L（P＜0.05），减少了心肌组织超氧化物的含量（P＜0.05）。
AVLE治疗显着降低gp91phox的表达（P＜0.05），使SOD活性增加（P＜0.05），MDA水平显著降低（P＜0.05）。结论：AVLE通过抑
制I/R心肌的氧化应激发挥心脏保护作用。 |
| 英文摘要: |
| Objective:To observe the effects of AVLE preconditioning on myocardial ischemia/reperfusion (MI/R) injury.Methods:Male Sprague-Dawley rats were divided into 3 groups randomly: sham, ischemia reperfusion and AVLE preconditioned group.
The model of myocardial I/R injury in vivo was made by ligating the left anterior descending artery for 30 min followed by 4 h of
reperfusion in SD rats. The hemodynamics were measured. The myocardial infarct size was measured by Evans blue and
2,3,5-triphenyltetrazolium chloride (TTC) staining. The level of hyperoxide superoxide, malondialdehyde (MDA) and plasma creatine
kinase (CK) were measured. The activities of antioxidant enzyme superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were
detected. The gp91phox were determined by Western Blot.Results:Compared with I/R group, the left ventricular ± dp/dtmax in AVLE
preconditioned rats was increased (P<0.05). The percentage of area of necrosis was reduced (32.0± 3.5% vs. 41.5± 4.5%, P<0.05). The
plasma CK and LDH levels were decreased to 1653± 62 U/L and 2461± 152 U/L (P<0.05 vs. MI/R group). AVLE preconditioning
decreased superoxide accumulation (P<0.05 vs. MI/R group). AVLE treatment markedly decreased gp91phox expression (P<0.05 vs. MI/R
group). The activity of SOD was increased (P<0.05 vs. MI/R group). The level of MDA was decreased (P<0.05 vs. MI/R group).Conclusion:AVLE alleviates I/R injury in myocardiumby inhibition of excessive ERS. |
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