| 陈砚芬 白军 徐家丽 宋晓晖 宁映霞.7-二氟甲氧基-5,4'-二正辛烷氧基金雀异黄素对人卵巢癌裸鼠移植瘤生
长的影响[J].,2015,15(22):4239-4243 |
| 7-二氟甲氧基-5,4'-二正辛烷氧基金雀异黄素对人卵巢癌裸鼠移植瘤生
长的影响 |
| Effects of 7-difluoromethoxyl-5,4'-di-n-octylygenistein on the Growth ofHuman Ovarian Carcinoma Xenografts in Nude Mice |
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| DOI： |
| 中文关键词: 卵巢癌 7- 二氟甲氧基-5，4'- 二正辛烷氧基金雀异黄素 移植瘤 凋亡 |
| 英文关键词: Ovarian cancer 7-difluoromethoxyl-5, 4'-di-n-octylygenistein Xenografts Apoptosis |
| 基金项目:国家自然科学青年基金项目(81301894) |
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| 中文摘要: |
| 目的：研究7- 二氟甲氧基-5,4'- 二正辛烷氧基金雀异黄素（7-difluoromethoxyl-5,4'-di-n-octylygenistein, DFOG）对人卵巢癌裸
鼠移植瘤生长的影响。方法：建立人卵巢癌HO-8910细胞裸鼠皮下移植瘤，随机分为5 组，每组5 只，即生理盐水组（Saline），紫杉
醇组（TAX），DFOG此处为3 组(5、10、20 mg/kg)组，观察各组移植瘤的体积和重量变化，观察各组荷瘤裸鼠重量及其血清乳酸脱
氢酶(LDH)、谷丙转氨酶(ALT)、肌酐值(Cr)、外周血白细胞计数(WBC)的变化。FCM检测移植瘤组织细胞的凋亡率，Western blot检
测移植瘤组织细胞cortactin 蛋白的表达变化。结果：DFOG各组显著抑制人卵巢癌HO-8910 细胞裸鼠移植瘤生长，与Saline 组比
较，移植瘤体积明显减小（P 均＜0.05），其中DFOG10mg/kg组对移植瘤抑制率与TAX 组相当（P＞0.05）；与Saline 组比较，DFOG
各组移植瘤重量明显减轻（P 均＜0.05），其中DFOG10mg/kg组对移植瘤重量减少率与TAX 组相当（P＞0.05）。与Saline 组及
DFOG 各组比较，TAX组荷瘤裸鼠重量显著降低（P均＜0.05），而Saline 组和DFOG 各组之间荷瘤裸鼠重量比较差异无统计学意
义（P＞0.05）；与Saline 组及DFOG 各组比较，TAX组荷瘤裸鼠血清LDH、ALT、Cr值和外周血WBC计数显著降低（P 均＞ 0.05），
而Saline 组与DFOG 各组比较，荷瘤裸鼠血清LDH、ALT、Cr 值和外周血WBC 计数无明显差异(P＞0.05)。DFOG 各组作用
HO-8910 细胞移植瘤16 d后，HO-8910 细胞发生凋亡，与Saline 组比较（P均＜0.05），其中DFOG10 mg/kg 组凋亡率与TAX 组相
当（P＞0.05）；同时cortactin 蛋白表达降低，与Saline 组比较（P 均＜0.05），其中DFOG10 mg/kg 组凋亡率与TAX 组相当（P＞
0.05）。结论：DFOG能抑制卵巢癌HO-8910 细胞裸鼠移植瘤生长，下调cortactin蛋白表达和诱导HO-8910 细胞凋亡。 |
| 英文摘要: |
| Objective:The aimof this study is to investigate the effects of 7-difluoromethoxyl-5, 4'-di-n-octylygenistein(DFOG) on
the growth of human ovarian carcinomaHO-8910 cells xenografts in nude mice.Methods:Models of human ovarian cancer HO-8910
cells xenografts transplanted subcutaneously in nude mice were established and randomly divided into five groups (each group including
five nude mice): Saline group, Taxol group, DFOG 5 mg/kg, DFOG 10 mg/kg and DFOG 20 mg/kg, the volume and weight of Xenograts
were observed and compared. The alteration of the weight of nude mice, and the change of serum levels of lactate dehydrogenase (LDH),
alanine aminotransferase (ALT), creatinine (Cr) and peripheral blood leukocyte counts (WBC counts) were examined and compared.
The cellular apoptotic rate of human ovarian carcinoma HO-8910 cells xenografts was analyzed by FCM. The expressions of cortactin
protein of HO-8910 cells xenografts were determined by western blot.Results:All DFOG groups could significantly suppress the
increasing volume of human ovarian carcinoma HO-8910 cells xenografts in nude mice models,there was dramatically statistical mean
compared with Saline group (Paverage＜0.05), and the inhibiting rate of DFOG10mg/kg was similar with TAX group (P＞0.05).
Compared with Saline group, the weight of Xenograts of DFOG groups dramatically decreased (Paverage＜0.05), and the inhibiting rate
of DFOG10mg/kg was almost equal to TAX group (P＞0.05). Compared with Saline group and each DFOG groups, the weight of
experimental nude mice of TAX group obviously decreased (Paverage＜0.05), while there was no statistical significance among Saline group and three DFOG groups (P＞0.05). Compared with Saline group and each DFOG groups, the serum levels of LDH, ALT, Cr and
WBC counts of experimental nude mice of TAX group significantly descended (Paverage＜0.05), while there was no difference among
Saline group and three DFOG groups (P＞0.05). When the xenografts were treated with DFOG for 16 days, xenograftscell tend to
apoptosis, there was difference compared with Saline group(Paverage＜0.05), and the apoptotic rate of xenografts cells of DFOG10mg/kg
was near to TAX group (P＞0.05). Simultaneously the expression of cortactin protein was down-regulated,and there were significantly
difference compared with Saline group (Paverage＜0.05), and thecortactin protein of xenografts cells of DFOG10mg/kg was similar to
TAX group (P＞0.05).Conclusion:DFOG could suppress the growth of human ovarian carcinoma HO-8910 cells xenografts in nude
mice, which may be related to induction of apoptosis through down-regulation expression of cortactin protein. |
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