| 符代炎 戴爱国 胡瑞成 田华 陈云荣 朱黎明.低氧诱导因子抑制因子在低氧性肺动脉高压中的作用[J].,2015,15(22):4263-4267 |
| 低氧诱导因子抑制因子在低氧性肺动脉高压中的作用 |
| Role of Factor Inhibiting Hypoxia-inducible Factor -1 in the Pathogenesis ofHypoxic Pulmonary Hypertension |
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| DOI： |
| 中文关键词: 肺动脉高压 低氧 低氧诱导因子抑制因子 肺小动脉 |
| 英文关键词: Pulmonary hypertension Hypoxia Factor inhibiting hypoxia-inducible factor-1 Pulmonary arterioles |
| 基金项目:国家自然科学基金项目（30971329，81270118）；湖南省自然科学基金项目（12JJ3111）；湖南省中医药科研计划项目（2013138） |
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| 中文摘要: |
| 目的：研究低氧性肺动脉高压（hypoxic pulmonary hypertension, HPH）形成过程中低氧诱导因子抑制因子（factor inhibiting
hypoxia-inducible factor -1, FIH）在肺小动脉的表达变化及在HPH 发病中的可能作用。方法：将36 名患者分为慢性阻塞性肺疾病
（chronic obstructive pulmonary disease, COPD）并肺动脉高压组（pulmonary hypertension, PH）组（PH 组）、COPD 非PH 组（COPD
组）、对照组，收集肺组织，观察其肺血管重塑指标，原位杂交法与免疫组织化学法检测肺小动脉壁FIH mRNA 和蛋白的表达水
平。结果：COPD 组患者肺小动脉出现血管重塑，PH 组患者肺小动脉重塑更明显（P＜0.05）。FIH mRNA在各组患者肺小动脉壁的
表达无明显差异（P＞0.05）；FIH蛋白在对照组患者肺小动脉壁高表达，COPD 组表达降低，PH组表达进一步减少（P＜0.05）。直
线相关分析表明，FIH蛋白与FIH mRNA 无相关（P＞ 0.05）；FIH蛋白与肺小动脉重塑指标、肺动脉收缩压均呈负相关（P＜ 0.01）。
结论：慢性肺泡性低氧下调患者肺小动脉壁FIH表达，进而参与患者HPH 发病。 |
| 英文摘要: |
| Objective:To investigate the dynamic expression of factor inhibiting hypoxia-inducible factor-1(FIH) in the pulmonary
arterioles of patients at different phases of hypoxic pulmonary hypertension (HPH) development, and the role of FIH in the pathogenesis
of HPH.Methods:Thirty-six patients were divided into three groups: chronic obstructive pulmonary disease (COPD) with pulmonary
hypertension (PH) group (PH group), COPD without PH (COPD group) and control group. Their lung tissues were collected from
surgically resected specimens. The pulmonary arteriole remodeling was observed with morphometric analysis. The expression of FIH in
pulmonary arterioles was examined by in situ hybridization and immunohistochemistry.Results:The pulmonary arteriole remodeling was
developed in patients from COPD group, and more significantly in patients from PH group (P＜0.05). The expression of FIH mRNA
remained unchanged in pulmonary arteriole walls between three groups (P＞0.05), while the expression of FIH protein was high in
pulmonary arteriole walls in control group, decreased markedly in COPD group and weakened further in PH group (P＜0.05). Linear
correlation analysis showed that there was no correlation between the FIH mRNA and FIH protein (P＞0.05). FIH protein was negatively
correlated with pulmonary arteriole remodeling parameters and pulmonary artery systolic pressure (P＜0.01).Conclusion:Under chronic
alveolar hypoxia, FIH is down-regulated in pulmonary arterioles, and may be involved in the pathogenesis of HPH in patients. |
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