| 王英磊 逄淑超 张莹莹 李忠海 李磊 孟琳 肖琳.TRF1、TRF2 和POT1 基因mRNA在前列腺癌组织中的表达[J].,2015,15(23):4518-4520 |
| TRF1、TRF2 和POT1 基因mRNA在前列腺癌组织中的表达 |
| Expressions of TRF1,TRF2 and POT1 Gene mRNA in Prostate Cancer |
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| DOI： |
| 中文关键词: 前列腺癌 端粒重复序列结合蛋白质1 端粒重复序列结合蛋白质2 端粒保护蛋白1 |
| 英文关键词: Prostate cancer TRF1 TRF2 POT1 |
| 基金项目:济宁市科技计划项目（2014jnjc15） |
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| 中文摘要: |
| 目的：探究端粒重复序列结合蛋白质1（TRF1）、TRF2 和端粒保护蛋白（POT1）基因mRNA 在前列腺癌(PCa)组织中的表达。
方法：收集46 例PCa 患者肿瘤中心组织（中心组织组）和35 例前列腺增生(BPH)患者BPH 组织（BPH 组织组），提取总RNA，逆
转录成cDNA，采用定量PCR测定TRF1、TRF2 和POT1 在中心组织组和BPH组织基因mRNA 的表达，采用基因mRNA所得
CT 值与茁-actin mRNA所得CT 值之差(△CT 值)表示，并进行差异性分析。结果：中心组织组TRF1 的△CT 值高于BPH 组织组,
差异有统计学意义(Z= -3.469，P =0.001)；TRF2 的△CT 值分别为8.49 (5.75，10.21)和8.16 (6.28，9.75)，差异无统计学意义(Z= -1.
719，P=0.086)；中心组织组POT1 的△CT 值高于BPH 组织组，差异有统计学意义(t= -18.48，P=0.000)。结论：TRF1 和POT1 在
PCa 肿瘤组织中的表达升高，说明TRF1和POT1的高表达可能与PCa 的发生和发展有关,但TRF2 在PCa 肿瘤中心组织和BPH
组织中的表达没有表现出差异，有待进一步研究。 |
| 英文摘要: |
| Objective:To explore the expression of telomeric repeat binding factor 1 (TRF1), factor 2 (TRF2) and protection of
telomeres1(POT1) gene mRNA in prostate cancer(PCa).Methods:A total of 46 cases of PCa center tissues(as center tissue group), and
35 cases of benign prostatic hyperplasia (BPH) tissues (as BPH tissue group) were collected and extracted the total RNA and transcribed
into cDNA in reverse. The gene mRNA expressions of TRF1, TRF2 and POT1 were detected by quantitative PCR method. The
difference (△CT value) between the value of CT of gene mRNA and the CT value of beta -actin mRNA was analysed.Results:The
△CT value of TRF1 in center tissue group was higher than that of BPH tissue group, and the difference was statistically significant(Z=
-3.469, P=0.001). The △CT values of TRF2 in center tissue group and BPH tissue group were 8.49(5.75, 10.21) and 8.16(6.28, 9.75), but
the difference was not statistically significant (Z= -1.719, P=0.086). The△CT value of POT1 in center tissue group was higher than that
of BPH tissue group, and the difference was statistically significant (t= -18.48, P=0.000).Conclusion:The high expression of TRF1 and
POT1 in PCa center tissue may be associated with the development and progression of PCa, but TRF2 shows no difference in PCa center
tissue and BPH tissue, which remains to be further studied. |
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