| 徐亦君 竺明晨 曹伟君 张振玉.UHRF1 在结肠癌中的表达及其与细胞增殖的关系[J].,2015,15(29):5628-5632 |
| UHRF1 在结肠癌中的表达及其与细胞增殖的关系 |
| The Expression of UHRF1 in Colon Cancer and the Relationship between ItsExpression with Cell Proliferation |
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| DOI： |
| 中文关键词: UHRF1 结肠癌 免疫组织化学 预后 增殖 |
| 英文关键词: UHRF1 Colon cancer Immunohistochemistry Prognosis Proliferation |
| 基金项目:江苏省卫生厅科研项目（H201325） |
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| 中文摘要: |
| 目的：探讨UHRF1 (Ubiquitin-like with plant homeodomain and ring finger domains 1)在结肠癌组织中的表达，及其与结肠癌
细胞增殖的关系。方法：采用免疫组织化学方法检测62 例结肠癌组织和癌旁正常对照组织的UHRF1表达，统计分析其表达和临
床病理资料和预后关系。采用siRNA抑制UHRF1 表达，分析其与细胞增殖的关系。结果：结肠癌组织中UHRF1高表达，癌旁正
常组织低表达。62 例结肠癌组织中33 例UHRF1 阳性，阳性率为53.2 %。UHRF1 表达与淋巴结转移、远处器官转移和Dukes' 分
期相关(P<0.05)。高表达UHRF1 结肠患者的生存时间明显较低表达者短(P<0.05)。单因素分析显示淋巴结转移、远处器官转移、
UHRF1 和Dukes' 分期与患者累积生存率相关(P<0.05)。多因素分析显示UHRF1 可作为结肠癌患者预后的独立危险因子(P<0.
05)。采用siRNA 转染HCT116 后，UHRF1 表达显著降低，细胞增殖被抑制。结论：UHRF1参与结肠癌细胞增殖，与结肠癌患者预
后密切相关。 |
| 英文摘要: |
| Objective:To investigate the expression of Ubiquitin-like with plant homeodomain and ring finger domains 1
(UHRF1) in colon cancer (CC) and the relationship between its expression and CC cell proliferation.Methods:Immunohistochemical
method was used to determine the expression of UHRF1 in 62 CC tissues and the adjacent normal tissues. The association of UHRF1
with clinicopathological features and prognoses was also analyzed. siRNA was utilized to evaluate the relationship between the expression
of UHRF1 and CC cell proliferation.Results:UHRF1 was overexpressed in 62 CC tissues, and expressed weak in the adjacent normal
tissues. Immunohistochemistry results showed that 33 of 62 cancer lesions (53.2 %) were stained positive, and high expression of
UHRF1 was correlated with the node metastasis, distant metastasis and Dukes' stage (P<0.05). The survival curve showed that patients
with high UHRF1 expression had significantly reduced overall survival rate (P<0.05). Univariate analysis of clinicopathologic parameters
revealed that overall survival rate was closely associated with UHRF1, node metastasis, distant metastasis and Dukes' stage (P<0.05).
Multivariate cox regression analysis indicated that UHRF1 was a prognostic marker for patients with CC (P<0.05). The expression of
UHRF1 was significantly reduced in HCT116 with siRNA transfection. Cell proliferation was inhibited in the siRNA group.Conclusion:UHRF1 is involved in the process of CC proliferation and closely associated with the prognoses of CC patients. |
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