| 肖景 孙云松 于俊生 姜超娜 徐平平 王荣.和络泄浊颗粒对大鼠肾间质纤维化miR-200a表达影响[J].,2015,15(30):5834-5838 |
| 和络泄浊颗粒对大鼠肾间质纤维化miR-200a表达影响 |
| Effect of Regulating Collateral and Eliminating Turbidity Granules on theExpression of miR-200a in Renal Interstitial Fibrosis in Rats |
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| DOI： |
| 中文关键词: 肾纤维化 和络泄浊颗粒 miR-200a |
| 英文关键词: Renal interstitial fibrosis Regulating Collateral and Eliminating Turbidity MiR-200a |
| 基金项目:山东省（2013-2014 年度）中医药科技发展计划项目（2013ZDZK-106） |
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| 中文摘要: |
| 目的：探索和络泄浊颗粒对大鼠肾脏纤维化中miR-200a 表达的影响及探讨其可能存在的作用机制。方法：30 只SD雄性大
鼠随机分为6 组：假手术组(Sham)、手术组(UUO)及和络泄浊颗粒(UUO+ REG)组各2 组，运用单侧（左）输尿管结扎法制造肾间质
纤维化模型，但Sham组仅游离输尿管，而其余两组则游离并结扎输尿管。术后各组按1 mg/kg·d-1的量进行灌胃，UUO+ REG组
给予REG，其余两组则予生理盐水。术后第7 天和14 天，摘除左梗阻侧肾脏进行免疫组化检查alpha-SMA和荧光定量PCR 检测
miR-200a 的表达。结果：在UUO及UUO+ REG 组，alpha-SMA 表达明显高于Sham 组（P＜0.01）；UUO+ REG 组低于UUO 组（P＜
0.05）。miR-200a 表达水平在UUO 组和UUO+ REG 组都是降低的，但是UUO 组与Sham 组差别明显（P＜0.01），其与UUO+
REG组亦有明显的差别（P＜0.05）。结论：和络泄浊颗粒可以通过上调miR-200a 延缓肾脏纤维化进展。 |
| 英文摘要: |
| Objective:To investigate the effects and mechanism of action of REG on microRNA-200a expression in renal
interstitial fibrosis rat that was established by unilateral ureter obstruction.Methods:Thirty male Sprague-Dawley (SD) rats were
randomly divided into six groups (5 mice/group): two shamoperation groups (Sham), two model groups (UUO), and two drug groups
(UUO+REG). Renal interstitial fibrosis was established in UUO and UUO+REG groups, but not in Sham groups, by unilateral ureter
obstruction. The left Kidney was harvested from all groups either 7 d or 14 d post-operation and subsequently examined for changes in
alpha-SMA or microRNA-200a expression.Results:The expression of alpha-SMA increased significantly (P < 0.01) in both UUO and
UUO+REG groups at 7 d and 14 d post-operation; however, there was a significant difference in alpha-SMA expression in UUO+REG group
compared with UUO group (P < 0.05). Finally, the expression levels of miR-200a were reduced in both UUO and UUO+REG groups as
compared with that in Shamgroups at 7 d and 14d post-operation, while the expression in UUO group was significantly lower than that in
Sham(P＜0.01) and UUO+REG groups（＜0.05）at 7d and 14 d post-operation.Conclusion:REG could delay the progression of chronic
renal failure by up-regulating miR-200a. |
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