| 王洁 温立婷 安鲁 李娟 陈福权 陈广生.丁酸钠器官毒性研究[J].,2015,15(31):6021-6023 |
| 丁酸钠器官毒性研究 |
| SodiumButyrate Toxicity Studies |
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| DOI： |
| 中文关键词: 丁酸钠 组蛋白去乙酰化酶抑制剂 毒性 |
| 英文关键词: Sodiumbutyrate HDAC inhibitor Toxicity |
| 基金项目:国家自然科学基金项目（81271069） |
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| 中文摘要: |
| 目的：研究组蛋白去乙酰化酶抑制剂(histone deacetylase inhibition HDACi)：丁酸钠（Sodium Butyrate NaB）腹腔连续给药对
BALB/C小鼠体重增长及器官发育的影响。方法：20 只健康，3 周BALB/C 小鼠随机分成2 组（丁酸钠组和对照组）；丁酸钠组腹腔
注射丁酸钠（NaB）1.2 g/ Kg·d，连续21 天；对照组同时间腹腔注射等量生理盐水。21 天后，测量体重；行4 %多聚甲醛灌注、固定，
取心脏，肝脏，脾脏，肺脏，肾脏，脑组织以及小肠组织器官，做石蜡切片，HE 染色两组比较有无器官损害。结果：1. 丁酸钠组与对
照组比较，两组动物体重增长良好，平均增长11 g，两组间无统计学差异（P>0.05）。2.HE 染色见丁酸钠组：心肌细胞无变性，坏死，
无炎性细胞浸润，无肉芽组织形成；脾脏红、白髓结构清晰，脾窦无扩张，未见炎性细胞浸润；肺间质无扩张，充血，未见纤维化，肺
泡无水肿；肾小管上皮细胞无变性坏死，肾间质未见水肿；脑细胞周围间隙和小血管间隙无增宽；肠道纤毛上皮排列整齐，肠壁无
出血，坏死，无渗出；肝细胞围绕中央静脉呈放射状排列，细胞无水肿，无胆汁淤积。未发现上述器官的病理变化。结论：丁酸钠长
期腹腔给药安全，无明显毒副作用。 |
| 英文摘要: |
| Objective:Research the organ toxicity of Histone deacetylase inhibition (HDACi); SodiumButyrate ( NaB ) through
the continuous iintraperitoneal injection in BALB/C mice.Methods:Twenty healthy, 3W BALB/C mice were randomly divided into 2
groups (NaB group and the control group), Intraperitoneal injection of NaB 1.2 g/Kg·d for 21 days (NaB group); intraperitoneal injection
of equivalent physiological saline for 21 days to be the control group. After measuring the weight gain, 4%paraformaldehyde glutaraldehyde
perfusion fixation, do the HE test of heart, liver, spleen, lung, kidney, brain tissue and intestinal tissue pathological slices.Results:1.
NaB group compared with the control group, average growth in 11 g. There is no statistical difference between the two groups(P>0.05).
2. The organ HE staining of NaB group. Cardiac muscle cell have no degeneration, necrosis, or inflammatory cell infiltration, and granulation
tissue formation; Spleen's red and white pulp structure is clear, there is no expansion of splenic sinus, and inflammatory cell infiltration;
Pulmonary interstitial have no expansion, congestion, or fibrosis, and no alveolar edema; Renal tubular epithelial cells have no
degeneration necrosis, renal interstitial have no edema. Brain cell's peripheral clearance and small vessel have no broaden; Intestinal ciliated
epithelium neatly, intestinal wall without hemorrhage, necrosis, and leakage. liver cells lie in a radial pattern around the central vein,
without edema or cholestasis. Did not observe any pathological changes on the above organ.Conclusion:Sodium butyrate intraperitoneal
injection is safe for a long time, and have no obvious side effects. |
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