| 汤晓飞 刘茂安 朱云祥△ 倪庆 周海.miR-96 通过RECK促进结直肠癌细胞的迁移和侵袭[J].,2015,15(33):6425-6428 |
| miR-96 通过RECK促进结直肠癌细胞的迁移和侵袭 |
| miR-96 Inhibitor Reduces Migration and Invasion of Colorectal Cancer Cellsvia RECK |
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| DOI： |
| 中文关键词: 结直肠癌 转移 miRNA-96 RECK |
| 英文关键词: Colorectal cancer Metastasis miR-96 RECK |
| 基金项目: |
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| 中文摘要: |
| 目的：研究miRNA-96 在结直肠癌转移中的作用及其机制。方法：采用Transwell 试验分析结直肠癌LoVo 细胞的迁移和侵
袭能力，采用荧光素报告基因及蛋白免疫印迹试验研究结直肠癌中miR-96的作用靶点。结果：miR-96 抑制剂处理后下调miR-96
的表达并抑制LoVo 细胞的迁移和侵袭。荧光素报告基因试验显示RECK是miR-96 的作用靶点，且RECK沉默能够部分阻碍
miR-96 抑制剂所导致的LoVo 细胞迁移和侵袭减少。结论：miRNA-96 可通过作用于RECK促进结直肠癌细胞转移，这可能成为
治疗结直肠癌转移的新靶点。 |
| 英文摘要: |
| Objective:To study the role and mechanisms of miR-96 in the metastasis of colorectal cancer (CRC).Methods:Transwell assays were performed to analyze the migration and invasion ability of CRC LoVo cells. Luciferase reporter assays and
western blot assays were used to research the direct target of miR-96 in CRC.Results:Down-regulation of miR-96 by inhibitor
suppressed the migration and invasion of LoVo cells. Luciferase reporter gene assay showed that RECK was a direct target of miR-96,
and RECK silencing could partially block the effect of miR-96 inhibitor in the migration and invasion of LoVo cells.Conclusion:MiR-96 could promote the metastasis of CRC through targeting RECK, which may act as a new target for the treatment of CRC
metastasis. |
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